Black R S, DeGiorgio L A, Sheu K F, Darzynkiewicz Z, Duffy J T, Blass J P
Altschul Laboratory for Dementia Research, Department of Neurology, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605.
J Neurochem. 1994 Jun;62(6):2132-40. doi: 10.1046/j.1471-4159.1994.62062132.x.
Cells have been cultured from the brains of 60-day-old rats and propagated through 12 passages. The cells contain the high and middle, but not low, molecular weight neurofilament subunits and neuron-specific enolase, demonstrated by immunoblotting and immunocytochemistry with redundant antibodies. The cells did not have the morphology of neurons when cultured in medium containing fetal calf serum and growth factors. In low serum medium containing the same growth factors with the addition of dibutyryl cyclic AMP, the cells became smaller and developed long processes. Three clonal lines derived from these cultures had the same properties. These observations are in agreement with recent observations using mouse and human brain tissue and demonstrate that proteins normally associated with neurons can be found in dividing cells cultured from the brains of young adult rats.
已从60日龄大鼠的大脑中培养出细胞，并传代12次。通过免疫印迹和使用多种抗体的免疫细胞化学方法证明，这些细胞含有高分子量和中分子量的神经丝亚基，但不含有低分子量的神经丝亚基以及神经元特异性烯醇化酶。当在含有胎牛血清和生长因子的培养基中培养时，这些细胞没有神经元的形态。在含有相同生长因子并添加二丁酰环磷腺苷的低血清培养基中，细胞变得更小并长出长突起。从这些培养物中获得的三个克隆系具有相同的特性。这些观察结果与最近使用小鼠和人类脑组织的观察结果一致，并表明在成年幼鼠大脑培养的分裂细胞中可以发现通常与神经元相关的蛋白质。
