Yoshikawa M, Chatani N, Harada E, Nishino Y, Yamahara J, Murakami N
Kyoto Pharmaceutical University, Japan.
Yakugaku Zasshi. 1994 Mar;114(3):176-81. doi: 10.1248/yakushi1947.114.3_176.
In order to characterize the chemical change of the constituents during the processing of Hydrangeae Dulcis Folium, quantitative analyses of phyllodulcin, hydrangenol, and their 8-O-glucosides were developed by means of high performance liquid chromatography. As an application of this HPLC method, the distribution of those dihydroisocoumarins in different parts of Hydrangea macrophylla var. thunbergii was investigated. It was found that these dihydroisocoumarins were contained at the highest concentration in the leaves. Furthermore, the seasonal fluctuation of these compounds in the leaves, together with the height of the plant and total dry weight of the leaves, were clarified and so that the suitable period for the harvest of Hydrangea macrophylla var. thunbergii was deduced to be from Oct. to Nov.
为了表征甜茶加工过程中成分的化学变化，采用高效液相色谱法对甜茶素、绣球酚及其8-O-葡萄糖苷进行了定量分析。作为该高效液相色谱法的应用，研究了这些二氢异香豆素在八仙花变种不同部位的分布。结果发现，这些二氢异香豆素在叶片中的含量最高。此外，还阐明了这些化合物在叶片中的季节性波动，以及植株高度和叶片总干重，并据此推断八仙花变种的适宜采收期为10月至11月。
