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预先存在的核结构决定了疱疹病毒DNA复制结构在核内的位置。

Preexisting nuclear architecture defines the intranuclear location of herpesvirus DNA replication structures.

作者信息

de Bruyn Kops A, Knipe D M

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

J Virol. 1994 Jun;68(6):3512-26. doi: 10.1128/JVI.68.6.3512-3526.1994.

DOI:10.1128/JVI.68.6.3512-3526.1994
PMID:8189490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC236855/
Abstract

Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have used the HSV ICP8 DNA-binding protein and bromodeoxyuridine labeling as markers for sites of herpesviral DNA synthesis to examine the spatial organization of these structures within the cell nucleus. Confocal microscopy and three-dimensional computer graphics reconstruction of optical series through infected cells indicated that viral DNA replication structures extend through the interior of the cell nucleus and appear to be spatially separate from the nuclear lamina. Examination of viral DNA replication structures in infected, binucleate cells showed similar or virtually identical patterns of DNA replication structures oriented along a twofold axis of symmetry between many of the sister nuclei. These results demonstrate that HSV DNA replication structures are organized in the interior of the nucleus and that their location is defined by preexisting host cell nuclear architecture, probably the internal nuclear matrix.

摘要

单纯疱疹病毒DNA复制蛋白定位于与受感染细胞核中病毒DNA复制结构相对应的特征性模式中。HSV DNA复制结构的核内空间组织以及调节其核定位的因素仍有待确定。我们使用HSV ICP8 DNA结合蛋白和溴脱氧尿苷标记作为疱疹病毒DNA合成位点的标志物，以检查这些结构在细胞核内的空间组织。通过感染细胞的光学系列进行共聚焦显微镜检查和三维计算机图形重建表明，病毒DNA复制结构延伸穿过细胞核内部，并且似乎在空间上与核纤层分开。对感染的双核细胞中的病毒DNA复制结构进行检查发现，许多姐妹细胞核之间沿二重对称轴定向的DNA复制结构模式相似或几乎相同。这些结果表明，HSV DNA复制结构在细胞核内部组织，其位置由预先存在的宿主细胞核结构（可能是内核基质）决定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/1eed7f39fa58/jvirol00015-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/a0816404023f/jvirol00015-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/ffa4dbd747dc/jvirol00015-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/e0c115122462/jvirol00015-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/b6e4d5190d56/jvirol00015-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/85e046e86aa9/jvirol00015-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/097c2e785dca/jvirol00015-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/7086256fde6a/jvirol00015-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/3cc259ce351f/jvirol00015-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/4bf5a0ace987/jvirol00015-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/1eed7f39fa58/jvirol00015-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/a0816404023f/jvirol00015-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/ffa4dbd747dc/jvirol00015-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/e0c115122462/jvirol00015-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/b6e4d5190d56/jvirol00015-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/85e046e86aa9/jvirol00015-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/097c2e785dca/jvirol00015-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/7086256fde6a/jvirol00015-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/3cc259ce351f/jvirol00015-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/4bf5a0ace987/jvirol00015-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8d8/236855/1eed7f39fa58/jvirol00015-0082-a.jpg

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