Carter K C, Bowman D, Carrington W, Fogarty K, McNeil J A, Fay F S, Lawrence J B
Department of Cell Biology, University of Massachusetts Medical Center,Worcester 01655.
Science. 1993 Feb 26;259(5099):1330-5. doi: 10.1126/science.8446902.
A quantitative three-dimensional analysis of nuclear components involved in precursor messenger RNA metabolism was performed with a combination of fluorescence hybridization, immunofluorescence, and digital imaging microscopy. Polyadenylate [poly(A)] RNA-rich transcript domains were discrete, internal nuclear regions that formed a ventrally positioned horizontal array in monolayer cells. A dimmer, sometimes strand-like, poly(A) RNA signal was dispersed throughout the nucleoplasm. Spliceosome assembly factor SC-35 localized within the center of individual domains. These data support a nuclear model in which there is a specific topological arrangement of noncontiguous centers involved in precursor messenger RNA metabolism, from which RNA transport toward the nuclear envelope radiates.
结合荧光杂交、免疫荧光和数字成像显微镜技术，对参与前体信使核糖核酸（mRNA）代谢的核成分进行了定量三维分析。富含聚腺苷酸[poly(A)]RNA的转录结构域是离散的核内区域，在单层细胞中形成腹侧定位的水平阵列。较弱的、有时呈链状的poly(A)RNA信号分散在整个核质中。剪接体组装因子SC-35定位于各个结构域的中心。这些数据支持了一种核模型，即参与前体mRNA代谢的非连续中心存在特定的拓扑排列，RNA从该排列向核膜辐射运输。
