Baev Vesselin, Iliev Ivan, Stefanov Yordan, Tsankova Marinela, Marhova Mariana, Apostolova Elena, Gozmanova Mariyana, Yahubyan Galina, Kostadinova Sonya
Department of Molecular Biology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, Bulgaria.
Department of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, Bulgaria.
Curr Issues Mol Biol. 2024 Mar 14;46(3):2497-2513. doi: 10.3390/cimb46030158.
Phospholipases find versatile applications across industries, including detergent production, food modification, pharmaceuticals (especially in drug delivery systems), and cell signaling research. In this study, we present a strain of for the first time, demonstrating significant potential in the production of phosphatidylcholine-specific phospholipase C (PC-PLC). The investigation thoroughly examines the PUMB_17 strain, focusing on the activity of PC-PLC and its purification process. Notably, the PUMB_17 strain displays extracellular PC-PLC production with high specific activity during the late exponential growth phase. To unravel the genetic makeup of PUMB_17, we employed nanopore-based whole-genome sequencing and subsequently conducted a detailed genome annotation. The genome comprises a solitary circular chromosome spanning 5,250,970 bp, featuring a guanine-cytosine ratio of 35.49. Additionally, two plasmids of sizes 64,250 bp and 5845 bp were identified. The annotation analysis reveals the presence of 5328 genes, encompassing 5186 protein-coding sequences, and 142 RNA genes, including 39 rRNAs, 103 tRNAs, and 5 ncRNAs. The aim of this study was to make a comprehensive genomic exploration that promises to enhance our understanding of the previously understudied and recently documented capabilities of and to shed light on a potential use of the strain in the industrial production of PC-PLC.
磷脂酶在多个行业有着广泛应用，包括洗涤剂生产、食品改性、制药（特别是在药物递送系统中）以及细胞信号研究。在本研究中，我们首次展示了一株在磷脂酰胆碱特异性磷脂酶C（PC-PLC）生产方面具有巨大潜力的菌株。该研究对PUMB_17菌株进行了全面考察，重点关注PC-PLC的活性及其纯化过程。值得注意的是，PUMB_17菌株在指数生长后期表现出具有高比活性的胞外PC-PLC生产能力。为了解PUMB_17的基因组成，我们采用了基于纳米孔的全基因组测序，随后进行了详细的基因组注释。该基因组包含一条单一的环状染色体，跨度为5,250,970 bp，鸟嘌呤-胞嘧啶比例为35.49。此外，还鉴定出了两个大小分别为64,250 bp和5845 bp的质粒。注释分析显示存在5328个基因，包括5186个蛋白质编码序列和142个RNA基因，其中包括39个rRNA、103个tRNA和5个非编码RNA。本研究的目的是进行全面的基因组探索，有望增进我们对该菌株此前研究不足且近期有文献记载的能力的理解，并揭示该菌株在PC-PLC工业生产中的潜在用途。
