Helset E, Kjaeve J, Hauge A
Department of Anesthesiology, University of Tromsø, Norway.
Circ Shock. 1993 Jan;39(1):15-20.
Effect of endothelin-1 (ET-1) (10(-8) M) on pulmonary microvascular permeability was examined in isolated rat lungs perfused with blood or various blood components. Microvascular permeability was assessed by measuring fluid filtration rate (FFR) in lungs pretreated with papaverine in order to prevent changes in vascular smooth muscle tone. ET-1 significantly increased FFR (131.0 +/- 10.1 mg/min, P < 0.01) after perfusion with blood for 60 min. In lungs perfused with leukocytes resuspended in plasma, ET-1 increased FFR significantly both 30 min (40.4 +/- 11.4 mg/min, P < 0.01) and 60 min (97.4 +/- 14.5 mg/min, P < 0.01) after it was added to the perfusate. Heat inactivation (56 degrees C; 1 hr) of plasma did not attenuate this effect of ET-1 (94.4 +/- 25.1 mg/min, P < 0.01). When lungs were perfused with leukocytes resuspended in Krebs Ringer albumin instead of plasma, or with plasma only, ET-1 did not cause any change in FFR. In conclusion, ET-1 increases microvascular permeability in isolated blood-perfused rat lungs. The effect is critically dependent on the presence of leukocytes and plasma components other than complement.
在灌注血液或各种血液成分的离体大鼠肺中，研究了内皮素 -1（ET-1）（10⁻⁸ M）对肺微血管通透性的影响。通过测量用罂粟碱预处理的肺中的液体滤过率（FFR）来评估微血管通透性，以防止血管平滑肌张力的变化。在用血液灌注60分钟后，ET-1显著增加了FFR（131.0±10.1毫克/分钟，P<0.01）。在灌注悬浮于血浆中的白细胞的肺中，在将ET-1添加到灌注液中30分钟（40.4±11.4毫克/分钟，P<0.01）和60分钟（97.4±14.5毫克/分钟，P<0.01）后，ET-1均显著增加了FFR。血浆的热灭活（56℃；1小时）并未减弱ET-1的这种作用（94.4±25.1毫克/分钟，P<0.01）。当用悬浮于 Krebs Ringer 白蛋白而非血浆中的白细胞灌注肺，或仅用血浆灌注时，ET-1未引起FFR的任何变化。总之，ET-1增加了离体血液灌注大鼠肺中的微血管通透性。该作用严重依赖于白细胞和补体以外的血浆成分的存在。
