Barry P H
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
J Neurosci Methods. 1994 Jan;51(1):107-16. doi: 10.1016/0165-0270(94)90031-0.
The JPCalc program has been designed to graphically illustrate how junction potential contributions arise in various electrophysiological situations, to enable the magnitude and direction of those values to be readily calculated and to show clearly how the resultant appropriate corrections need to be applied to experimental measurements. In particular, the program covers the analysis of junction potential contributions and corrections in all the various different patch-clamp configurations (intact patches, inside-out patches, outside-out patches, whole-cell recording and perforated patches), intracellular recording, epithelial and bilayer measurements. It can also be used to correct experimental measurements designed to try to directly measure junction potentials and to compare both the magnitude and direction of such corrected measurements with their theoretical values. Although it is recommended that all of the above measurements be done with salt-filled electrodes or salt bridges, the program will allow the use of Ag/AgCl reference electrodes in direct contact with bathing solution in most situations. JPCalc also makes use of a library file of mobility and valency data for about 30 ions which can be extended by the user. In addition to its value in correcting electrical measurements, the program with its graphical displays may also be of value in focusing discussion about the contribution of junction potentials to such measurements and in helping to design experiments to either minimize their contribution or to measure their values. The JPCalc program, designed for IBM-compatible computers with color or monochrome monitors, can also be run on Macintosh computers with SoftPC emulation software.
JPCalc程序旨在以图形方式说明在各种电生理情况下结电位贡献是如何产生的，以便能够轻松计算这些值的大小和方向，并清楚地显示如何对实验测量应用相应的校正。特别是，该程序涵盖了所有不同膜片钳配置（完整膜片、内向外膜片、外向内膜片、全细胞记录和穿孔膜片）、细胞内记录、上皮和双层测量中的结电位贡献和校正分析。它还可用于校正旨在直接测量结电位的实验测量，并将此类校正测量的大小和方向与其理论值进行比较。尽管建议上述所有测量都使用充满盐的电极或盐桥进行，但在大多数情况下，该程序允许使用与浴液直接接触的Ag/AgCl参比电极。JPCalc还利用了一个包含约30种离子迁移率和价态数据的库文件，用户可以对其进行扩展。除了在校正电学测量方面的价值外，该程序及其图形显示在聚焦关于结电位对此类测量的贡献的讨论以及帮助设计实验以最小化其贡献或测量其值方面也可能具有价值。JPCalc程序专为配备彩色或单色显示器的IBM兼容计算机设计，也可在装有SoftPC仿真软件的Macintosh计算机上运行。
