Ng B, Barry P H
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
J Neurosci Methods. 1995 Jan;56(1):37-41. doi: 10.1016/0165-0270(94)00087-w.
Junction potential corrections are often required in electrophysiological measurements, particularly when using the patch-clamp technique. The ionic conductivities and mobilities of certain less common organic ions were measured for use in junction potential corrections in electrophysiological measurements. The mobilities of these ions relative to K+ were: aspartate (0.30), choline (0.51), gluconate (0.33), glutamate (0.26), HEPES (0.30), isethionate (0.52), MES (0.37), MOPS (0.35), N-methyl-D-glucamine (0.33), and Tris (0.40). In addition, junction potentials were directly measured for a number of typical simplified patch-clamp solutions containing these ions. The measured junction potentials generally agreed well with the values calculated using the Generalized Henderson Equation with the appropriate ionic mobility. The accuracy of the measurements is discussed.
在电生理测量中，尤其是使用膜片钳技术时，通常需要进行液接电位校正。测量了某些不太常见的有机离子的离子电导率和迁移率，以用于电生理测量中的液接电位校正。这些离子相对于K⁺的迁移率分别为：天冬氨酸（0.30）、胆碱（0.51）、葡萄糖酸盐（0.33）、谷氨酸（0.26）、HEPES（0.30）、羟乙基磺酸（0.52）、MES（0.37）、MOPS（0.35）、N-甲基-D-葡糖胺（0.33）和Tris（0.40）。此外，还直接测量了许多含有这些离子的典型简化膜片钳溶液的液接电位。测得的液接电位通常与使用广义亨德森方程并结合适当离子迁移率计算得到的值非常吻合。文中讨论了测量的准确性。
