Piechowicz L, Namysł E, Galiński J
Katedra i Zakład Mikrobiologii Lekarskiej AM, Gdaánsku.
Med Dosw Mikrobiol. 1993;45(3):273-6.
The investigation was performed on 923 strains of S. aureus isolated from clinical material obtained from several regions of Poland. Resistance to methicillin was tested by a dilution method on a solid Mueller-Hinton medium supplemented with 2% of NaCl. Strains exhibiting MIC higher than 4 micrograms/ml were determined as resistant. Resistance to other antibiotics (P, Am, CB, CF, CH, MA, Ge, Bs, E, L, Dx) was tested by a disc method. Bacteriophage typing of S. aureus was performed by a method described by Blair and Williams in RTD and RTD x 100, using basic set of phages and additional phages (88, 89, 187). MRSA were present in various regions of the country with similar frequency (from 3.3% to 8.3%). In one center only the percentage was as high as 52.3%. High percentage of MRSA was noted in burned patients (59.9) and these strains were obtained at one center. Within the MRSA III phage group was dominating as well as non-typable strains and inhibited at 100 x RTD-35.4%. MRSA most frequently were typing with phages 88-40.9%, 89-35.0%, 85-24.0%, and they rarely belonged to the phage group II. Among MRSA strains higher percentage of antibiotic-resistance was noted, as compared with other strains. About 60% of MRSA strains were resistant to 6-8 antibiotics. The dominating resistance concerned penicillins, cephalosporins, aminoglycosides, tetracyclines and erythromycin.
对从波兰多个地区获取的临床材料中分离出的923株金黄色葡萄球菌进行了调查。在添加2%氯化钠的固体Mueller-Hinton培养基上，通过稀释法检测对甲氧西林的耐药性。将最低抑菌浓度（MIC）高于4微克/毫升的菌株判定为耐药。通过纸片法检测对其他抗生素（青霉素、氨苄西林、羧苄西林、头孢噻吩、头孢唑林、苯唑西林、庆大霉素、杆菌肽、红霉素、林可霉素、多西环素）的耐药性。金黄色葡萄球菌的噬菌体分型采用Blair和Williams描述的方法，在常规试验剂量（RTD）和RTD×100下进行，使用基本噬菌体组合和额外的噬菌体（88、89、187）。耐甲氧西林金黄色葡萄球菌（MRSA）在该国不同地区的出现频率相似（从3.3%到8.3%）。仅在一个中心该百分比高达52.3%。在烧伤患者中发现高比例的MRSA（59.9%），且这些菌株来自同一个中心。在MRSA中，III型噬菌体组占主导地位，还有不可分型菌株，在100×RTD时受到抑制的比例为35.4%。MRSA最常被88型噬菌体分型（40.9%）、89型噬菌体分型（35.0%）、85型噬菌体分型（24.0%），它们很少属于II型噬菌体组。与其他菌株相比，MRSA菌株中抗生素耐药的比例更高。约60%的MRSA菌株对6 - 8种抗生素耐药。主要的耐药涉及青霉素、头孢菌素、氨基糖苷类、四环素和红霉素。
