Wiśniewska U, Galiński J
Katedra i Zakład Mikrobiologii Lekarskiej AM, Gdańsku.
Med Dosw Mikrobiol. 1993;45(3):277-9.
Investigated 3558 strains of S. aureus were obtained from healthy newborn infants in neonatal wards. The staphylococci were classified as S. aureus on the basis of a positive coagulase test. Bacteriophage typing was performed with a basic set of phages and an additional phage (187), according to the method Blair and Williams, in RTD and RTD x 100. Additionally, susceptibility of staphylococcal phagotypes isolated in the years 1988-1991 to antibiotic from penicillin and cephalosporin groups, trimethoprim-sulphamethoxazole, erythromycin, lincomycin and doxycycline, was determined by disc diffusion method. Among tested strains phagotypes of group II dominated. They constituted 31.3% of all present phagotypes. Second in frequency were phagotypes of group V and III (13.2% and 10.0%). Large number of staphylococci (22.9%) was typable at the same time with phages belonging to different phage groups. Tested staphylococci were highly resistant to penicillin (98.8%), ampicillin (98.9%), cloxacillin (38.6%), carbenicillin (32.6%) and doxycycline (45.6%).
从新生儿病房的健康新生儿中获取了3558株金黄色葡萄球菌菌株。根据凝固酶试验阳性将葡萄球菌分类为金黄色葡萄球菌。按照布莱尔和威廉姆斯的方法,使用一组基本噬菌体和一种额外的噬菌体(187),在常规试验剂量(RTD)和RTD×100下进行噬菌体分型。此外,通过纸片扩散法测定了1988 - 1991年分离出的葡萄球菌噬菌体类型对青霉素类、头孢菌素类抗生素、甲氧苄啶 - 磺胺甲恶唑、红霉素、林可霉素和强力霉素的敏感性。在受试菌株中,II组噬菌体类型占主导。它们占所有现有噬菌体类型的31.3%。其次是V组和III组噬菌体类型(分别为13.2%和10.0%)。大量葡萄球菌(22.9%)可同时被不同噬菌体组的噬菌体分型。受试葡萄球菌对青霉素(98.8%)、氨苄青霉素(98.9%)、氯唑西林(38.6%)、羧苄青霉素(32.6%)和强力霉素(45.6%)高度耐药。