Jerant-Patić V, Ziramov J, Vujkov V, Mrda E, Hrnjaković-Cvjetković I, Milosević V
Pokrajinski zavod za zdravstvenu zastitu, Medicinski fakultet, Novi Sad.
Med Pregl. 1993;46(5-6):209-12.
A sample of 596 women was examined by the direct immunofluorescent test (DIF) for the detection of chlamydia brachomatis antigens in the endocervical smear (with monoclonal antibodies), ELISA test for the detection of IgG antibodies against the group chlamydia-antigen. The chlamydia trachomatis infection was found in 30.20% of the cases, with a high positive rate in all age groups. These infections were more frequently found in women in whom sterility had been diagnosed, compared to other examined women. In relation to other inflammatory processes the significantly highest rate was found in women with changes in the cervix (endocervicitis and arythroplakia). A high rate of chlamydia positive (42.44%) was detected in women who had had pathological pregnancies and deliveries. Humoral immune response to the presence of chlamydia trachomatis infection was proved in 79.44% of the cases. We showed and analyzed diagnostic possibilities of the used diagnostic tests where a combination of the DIF test and ELISA was found the best. For a successful diagnosis two methods have been recommended: a direct one-for the detection of the cause (DIF test, isolation in the cell culture McCoy or Hela 229) and an indirect one for the detection of specific antibodies in the serum.
对596名女性样本进行了直接免疫荧光试验（DIF）（使用单克隆抗体检测宫颈涂片中的沙眼衣原体抗原）、酶联免疫吸附测定（ELISA）试验（检测针对衣原体属抗原的IgG抗体）。在30.20%的病例中发现了沙眼衣原体感染，所有年龄组的阳性率都很高。与其他接受检查的女性相比，这些感染在已诊断为不育的女性中更为常见。与其他炎症过程相关，宫颈有病变（宫颈内膜炎和红斑）的女性中感染率显著最高。有过病理妊娠和分娩史的女性中，衣原体阳性率很高（42.44%）。79.44%的病例证实了对沙眼衣原体感染存在体液免疫反应。我们展示并分析了所用诊断试验的诊断可能性，发现DIF试验和ELISA联合使用效果最佳。为了成功诊断，推荐两种方法：一种是直接检测病因（DIF试验，在McCoy细胞培养物或Hela 229细胞中分离），另一种是间接检测血清中的特异性抗体。
