大鼠附睾脂肪垫和分离脂肪细胞线粒体中的可交换钙池与总钙池。在丙酮酸脱氢酶活性调节中的作用。
Exchangeable and total calcium pools in mitochondria of rat epididymal fat-pads and isolated fat-cells. Role in the regulation of pyruvate dehydrogenase activity.
作者信息
Severson D L, Denton R M, Bridges B J, Randle P J
出版信息
Biochem J. 1976 Jan 15;154(1):209-23. doi: 10.1042/bj1540209.
Abstract
- Isolated fat-cells and intact epididymal fat-pads were incubated in medium containing 45Ca2+ and the incorporation of 45Ca into mitochondrial and extramitochondrial fractions was studied. Redistribution of 45Ca between these fractions was essentially prevented by the addition of EGTA [ethanedioxybis(ethylamine)tetra-acetate] and Ruthenium Red to the sucrose-based extraction medium. 2. Incorporation of 45Ca into mitochondrial fractions of both fat-cells and fat-pads was found to be complete within 2-5 min, suggesting that mitochondria contain a pool of calcium in rapid isotopic exchange with extracellular Ca2+. This pool was about 20 times larger in mitochondria within fat-cells than within fat-pads. In fat-cells, 45Ca incorporation into the mitochondrial fraction accounted for about 34% of the total 45Ca incorporation into cells after 20 min and about 50% of the total mitochondrial calcium content measured by atomic absorption; values in fat-pads were about 7 and 20% respectively.
摘要
- 将分离的脂肪细胞和完整的附睾脂肪垫置于含有45Ca2+的培养基中进行孵育，并研究45Ca在线粒体和线粒体外部分的掺入情况。通过向基于蔗糖的提取培养基中添加乙二醇双（乙胺）四乙酸（EGTA）和钌红，基本上阻止了45Ca在这些部分之间的重新分布。2. 发现脂肪细胞和脂肪垫的线粒体部分在2 - 5分钟内完全掺入45Ca，这表明线粒体含有一个与细胞外Ca2+进行快速同位素交换的钙池。脂肪细胞内线粒体中的这个钙池比脂肪垫内的大约大20倍。在脂肪细胞中，20分钟后，45Ca在线粒体部分的掺入量占细胞总45Ca掺入量的约34%，占通过原子吸收测量的线粒体总钙含量的约50%；脂肪垫中的相应值分别约为7%和20%。
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