Itoh Y, Kitamura Y, Fukazawa C
Division of Applied Microbiology, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Mol Gen Genet. 1994 May 10;243(3):353-7. doi: 10.1007/BF00301071.
The soybean embryo factor binding sequence in the glycinin A2B1a gene promoter was delimited to an A/T-rich 9 bp sequence, 5'-TAATAATTT-3', designated as the glycinin box, by DNA footprinting and gel mobility shift assay using synthetic oligonucleotides. It was shown that the interaction with the factor takes place at a defined DNA sequence rather than at random A/T-rich sequence blocks in the glycinin 5' flanking region. There are four glycinin boxes in the quantitative regulatory region between positions -545 and -378 of the glycinin A2B1a promoter. Multiple nonamer motifs similar to the glycinin box were also found in the equivalent regions of other glycinin and legumin promoters, suggesting that they must be conserved as a binding site for the embryo factor that activates the differential and stage-specific expression of seed 11S globulin genes in leguminous plants.
通过使用合成寡核苷酸的DNA足迹法和凝胶迁移率变动分析，大豆球蛋白A2B1a基因启动子中的大豆胚胎因子结合序列被限定为一个富含A/T的9碱基序列，即5'-TAATAATTT-3'，被命名为大豆球蛋白盒。结果表明，与该因子的相互作用发生在特定的DNA序列上，而不是在大豆球蛋白5'侧翼区域中随机的富含A/T的序列块上。在大豆球蛋白A2B1a启动子-545至-378位之间的定量调控区域中有四个大豆球蛋白盒。在其他大豆球蛋白和豆球蛋白启动子的等效区域中也发现了多个与大豆球蛋白盒相似的九聚体基序，这表明它们作为胚胎因子的结合位点必须是保守的，该胚胎因子可激活豆科植物种子11S球蛋白基因的差异表达和阶段特异性表达。
