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1
P2 functions as a spacer in the Tetrahymena ribozyme.P2在四膜虫核酶中起间隔序列的作用。
Nucleic Acids Res. 1994 Apr 25;22(8):1383-8. doi: 10.1093/nar/22.8.1383.
2
A shortened form of the Tetrahymena thermophila group I intron can catalyze the complete splicing reaction in trans.嗜热四膜虫I组内含子的一种缩短形式可以反式催化完整的剪接反应。
J Mol Biol. 1993 Oct 20;233(4):629-43. doi: 10.1006/jmbi.1993.1541.
3
A tertiary interaction in the Tetrahymena intron contributes to selection of the 5' splice site.嗜热四膜虫内含子中的三级相互作用有助于5'剪接位点的选择。
Genes Dev. 1994 May 15;8(10):1198-211. doi: 10.1101/gad.8.10.1198.
4
Mutations in the Tetrahymena ribozyme internal guide sequence: effects on docking of the P1 helix into the catalytic core and correlation with catalytic activity.嗜热四膜虫核酶内部引导序列中的突变：对P1螺旋对接至催化核心的影响及其与催化活性的相关性。
Biochemistry. 1996 Sep 3;35(35):11493-502. doi: 10.1021/bi960510z.
5
RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme.嗜热四膜虫核酶催化核心中的RNA底物结合位点。
Nature. 1992 Jul 9;358(6382):123-8. doi: 10.1038/358123a0.
6
RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron.RNA的结构而非序列决定了I类内含子的5'剪接位点特异性。
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7402-6. doi: 10.1073/pnas.86.19.7402.
7
Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA-RNA interaction.通过非天然RNA-RNA相互作用对嗜热四膜虫I组内含子核酶进行反式激活。
Nucleic Acids Res. 1999 Apr 1;27(7):1650-5. doi: 10.1093/nar/27.7.1650.
8
Trans-activation of the Tetrahymena ribozyme by its P2-2.1 domains.四膜虫核酶通过其P2-2.1结构域的反式激活。
J Biochem. 1998 Mar;123(3):528-33. doi: 10.1093/oxfordjournals.jbchem.a021968.
9
Two universally conserved adenosines of the group I intron that are important for self-splicing but not for core catalytic activity.I组内含子的两个普遍保守的腺苷，它们对自我剪接很重要，但对核心催化活性不重要。
J Biochem. 1994 Jan;115(1):126-30. doi: 10.1093/oxfordjournals.jbchem.a124286.
10
Optimizing the substrate specificity of a group I intron ribozyme.优化I组内含子核酶的底物特异性。
Biochemistry. 1999 Mar 16;38(11):3426-32. doi: 10.1021/bi982688m.

引用本文的文献

1
Group I introns and GNRA tetraloops: remnants of 'The RNA world'?I类内含子与GNRA四环：“RNA世界”的遗迹？
Mol Biol Rep. 2008 Jun;35(2):239-49. doi: 10.1007/s11033-007-9076-4. Epub 2007 Apr 17.
2
A peripheral element assembles the compact core structure essential for group I intron self-splicing.一个外围元件组装了I组内含子自我剪接所必需的紧密核心结构。
Nucleic Acids Res. 2005 Aug 12;33(14):4602-11. doi: 10.1093/nar/gki770. Print 2005.

本文引用的文献

1
Sequence of a ribosomal RNA gene intron from Tetrahymena.来自四膜虫的核糖体RNA基因内含子序列。
Nature. 1980 Feb 14;283(5748):693-4. doi: 10.1038/283693a0.
2
Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.嗜热四膜虫核糖体RNA间隔序列的二级结构：与真菌线粒体间隔序列的结构同源性。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3903-7. doi: 10.1073/pnas.80.13.3903.
3
Sequence requirements for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA.嗜热栖热菌前核糖体RNA自我剪接的序列要求。
Nucleic Acids Res. 1985 Mar 25;13(6):1871-89. doi: 10.1093/nar/13.6.1871.
4
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.使用T7 RNA聚合酶和合成DNA模板进行寡核糖核苷酸合成。
Nucleic Acids Res. 1987 Nov 11;15(21):8783-98. doi: 10.1093/nar/15.21.8783.
5
Structural conventions for group I introns.第一类内含子的结构规则。
Nucleic Acids Res. 1987 Sep 25;15(18):7217-21. doi: 10.1093/nar/15.18.7217.
6
Structural conservation among three homologous introns of bacteriophage T4 and the group I introns of eukaryotes.噬菌体T4的三个同源内含子与真核生物I类内含子之间的结构保守性。
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1151-5. doi: 10.1073/pnas.85.4.1151.
7
Enzymatic activity of the conserved core of a group I self-splicing intron.I类自我剪接内含子保守核心的酶活性
Nature. 1986;322(6074):83-6. doi: 10.1038/322083a0.
8
A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii.卡氏肺孢子虫小亚基核糖体RNA基因中的一个自我剪接内含子。
Nucleic Acids Res. 1989 Jul 11;17(13):5349-59. doi: 10.1093/nar/17.13.5349.
9
RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron.RNA的结构而非序列决定了I类内含子的5'剪接位点特异性。
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7402-6. doi: 10.1073/pnas.86.19.7402.
10
Catalytic activity is retained in the Tetrahymena group I intron despite removal of the large extension of element P5.尽管P5元件的大片段延伸被去除，但嗜热四膜虫I组内含子仍保留催化活性。
Nucleic Acids Res. 1989 Oct 11;17(19):7879-89. doi: 10.1093/nar/17.19.7879.

P2在四膜虫核酶中起间隔序列的作用。

P2 functions as a spacer in the Tetrahymena ribozyme.

作者信息

Peyman A

机构信息

Hoechst AG, Allgemeine Pharma Forschung, Frankfurt, Germany.

出版信息

Nucleic Acids Res. 1994 Apr 25;22(8):1383-8. doi: 10.1093/nar/22.8.1383.

DOI:10.1093/nar/22.8.1383
PMID:8190628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307994/
Abstract

The function of the P2 stem-loop region in the group I catalytic intron from Tetrahymena thermophila has been investigated. A comprehensive mutation analysis suggests that the bottom base pair of the P2 stem and nucleotides in the loop L2 are involved in interactions elsewhere on the intron. In addition, the P2 stem can be varied only between 9 and 11 base pairs in length. Phylogenetic evidence (3) from a sub-class of group I introns supports a model in which the P1 and P2 stems are coaxially stacked. We found that variation of the length of P2 does not shift the sites of intron-catalyzed cleavage in P1 (9). This suggests that coaxial stacking of the P1 and P2 stems is unlikely in the Tetrahymena intron. A narrowing of the window for cleavage activity and a drop in cleavage efficiency are observed when substrates with an insertion in P2 are compared with those with a deletion. A possible explanation for this phenomenon is an unfavorable movement of P1 away from the active site as a result of the the lengthening of P2.

摘要

嗜热四膜虫第一类催化内含子中P2茎环区域的功能已得到研究。全面的突变分析表明，P2茎的底部碱基对和环L2中的核苷酸参与了内含子其他部位的相互作用。此外，P2茎的长度只能在9到11个碱基对之间变化。来自第一类内含子一个亚类的系统发育证据支持P1和P2茎同轴堆积的模型。我们发现P2长度的变化不会改变P1中内含子催化切割的位点（9）。这表明在嗜热四膜虫内含子中，P1和P2茎同轴堆积的可能性不大。当将P2中有插入的底物与有缺失的底物进行比较时，观察到切割活性窗口变窄且切割效率下降。对此现象的一种可能解释是，由于P2延长，P1会向远离活性位点的方向发生不利移动。