Wild M A, Sommer R
Nature. 1980 Feb 14;283(5748):693-4. doi: 10.1038/283693a0.
Recently, several genes coding for messenger RNA, transfer RNA and ribosomal RNA in eukaryotes have been found to be interrupted in their coding regions by DNA sequences which are not represented in the mature RNA transcripts (see ref. 1 for review). Many of these intervening sequences, or introns, are now known to be transcribed in the precursor RNA, from which they are subsequently processed out to form the mature RNA. As the intron-exon junctions must in some way be recognised for accurate splicing, the nucleotide sequences of these regions from a number of protein-coding and tRNA genes have been analysed. Sequence homologies were found at the splice points of the protein-coding gene introns from diverse organisms, but the tRNA intron boundaries were not similar to these. This has led to the speculation that different splicing activities are necessary for the processing of introns in mRNA and tRNA precursors. We report here the sequence of a ribosomal RNA gene intron from Tetrahymena in which intron-exon junctions differ from those analysed to date.
最近，人们发现真核生物中一些编码信使RNA、转运RNA和核糖体RNA的基因，其编码区域被一些在成熟RNA转录本中不存在的DNA序列所中断（综述见参考文献1）。现在已知许多这些间隔序列，即内含子，会在前体RNA中被转录，随后从中被加工去除以形成成熟RNA。由于内含子 - 外显子连接点必须以某种方式被识别才能进行准确的剪接，因此对许多蛋白质编码基因和tRNA基因这些区域的核苷酸序列进行了分析。在来自不同生物体的蛋白质编码基因内含子的剪接位点发现了序列同源性，但tRNA内含子边界与这些不同。这引发了一种推测，即mRNA和tRNA前体中内含子的加工需要不同的剪接活性。我们在此报告来自嗜热栖热菌的一个核糖体RNA基因内含子的序列，其中内含子 - 外显子连接点与迄今为止分析的不同。
