代表嗜盐古菌Halobacterium sp. GRB基因组的物理图谱和一组重叠黏粒克隆。

St Jean A, Trieselmann B A, Charlebois R L

Department of Biology, University of Ottawa, Ontario, Canada.

Nucleic Acids Res. 1994 Apr 25;22(8):1476-83. doi: 10.1093/nar/22.8.1476.

We have constructed a complete, five-enzyme restriction map of the genome of the archaeon Halobacterium sp. GRB, based on a set of 84 overlapping cosmid clones. Fewer than 30 kbp, in three gaps, remain uncloned. The genome consists of five replicons: a chromosome (2038 kbp) and four plasmids (305, 90, 37, and 1.8 kbp). The genome of Halobacterium sp. GRB is similar in style to other halobacterial genomes by being partitioned among multiple replicons and by being mosaic in terms of nucleotide composition. It is unlike other halobacterial genomes, however, in lacking multicopy families of insertion sequences.

我们基于一组84个重叠黏粒克隆构建了嗜盐古菌盐杆菌属GRB菌株基因组的完整五酶切限制图谱。在三个间隙中，未克隆的片段少于30千碱基对。该基因组由五个复制子组成：一条染色体（2038千碱基对）和四个质粒（305、90、37和1.8千碱基对）。盐杆菌属GRB菌株的基因组在结构上与其他嗜盐细菌基因组相似，即由多个复制子组成，并且在核苷酸组成方面呈镶嵌状。然而，它与其他嗜盐细菌基因组不同的是，缺乏插入序列的多拷贝家族。