López-García P, St Jean A, Amils R, Charlebois R L
Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
J Bacteriol. 1995 Mar;177(5):1405-8. doi: 10.1128/jb.177.5.1405-1408.1995.
Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-point chromosomal comparison revealed only two inversions and a few translocations. Forces known to promote rearrangement, common in the haloarchaea, have been ineffective in changing global gene order throughout the nearly 10(7) years of these species' divergent evolution.
通过可用探针的杂交,我们已将9个基因添加到地中海嗜盐菌染色体的宏观限制图谱中,并将5个基因添加到沃氏嗜盐菌的重叠群图谱中。此外,我们将沃氏嗜盐菌17个已定位的黏粒克隆与地中海嗜盐菌基因组进行了杂交。由此产生的35点染色体比较仅揭示了两个倒位和一些易位。已知在嗜盐古菌中常见的促进重排的力量,在这些物种近10^7年的分化进化过程中,在改变全球基因顺序方面并未发挥作用。