Rowlands T, Baumann P, Jackson S P
Wellcome/CRC Institute, Cambridge, UK.
Science. 1994 May 27;264(5163):1326-9. doi: 10.1126/science.8191287.
The TATA-binding protein TBP appears to be essential for all transcription in eukaryotic cell nuclei, which suggests that its function was established early in evolution. Archaebacteria constitute a kingdom of organisms distinct from eukaryotes and eubacteria. Archaebacterial gene regulatory sequences often map to TATA box-like motifs. Here it is shown that the archaebacterium Pyrococcus woesei expresses a protein with structural and functional similarity to eukaryotic TBP molecules. This suggests that TBP's role in transcription was established before the archaebacterial and eukaryotic lineages diverged and that the transcription systems of archaebacteria and eukaryotes are fundamentally homologous.
TATA 结合蛋白 TBP 似乎对真核细胞核中的所有转录过程都至关重要，这表明其功能在进化早期就已确立。古细菌构成了一个与真核生物和真细菌不同的生物界。古细菌的基因调控序列常常定位到类似 TATA 框的基序上。本文表明，古细菌沃氏嗜热栖热菌表达一种与真核 TBP 分子在结构和功能上相似的蛋白质。这表明 TBP 在转录中的作用在古细菌和真核生物谱系分化之前就已确立，并且古细菌和真核生物的转录系统在根本上是同源的。
