Balzi M, Becciolini A, Mauri P, Larosa V, Bechi P
Department of Clinical Physiopathology, University of Florence, Italy.
In Vivo. 1993 Nov-Dec;7(6B):635-7.
The study deals with the analysis of proliferative activity in colon carcinomas and adjacent normal appearing mucosa, evaluated with in vitro 3H-Thymidine and autoradiography. In the colonic mucosa no significant differences in 3H-Thymidine Labelling Index (TLI) were observed in relation to the distance of the sample from the neoplasia. The distribution of S-phase cells along the crypt length is low at the bottom, increases rapidly with a maximum within the lower 25% and decreases in the highest positions. When the proliferative activity is increased there is the possibility of expanding the proliferative compartment towards the luminal region of the crypt. The division of the crypt into 5 parts makes it possible to identify 2 different patterns: the first with a very high TLI in the lower fifth, then a sharp decrease and without labelled cells in the highest parts; the second with labelled cells present also in the luminal fifth. These 2 aspects are characteristic of specimens with the lowest and the highest TLI values respectively. The analysis of TLI in colo-rectal cancers shows that cell kinetics parameters are not related to clinical and histopathological features such as sex, age, Dukes and TNM stages and grade of differentiation.
本研究采用体外³H-胸腺嘧啶核苷和放射自显影技术，对结肠癌及相邻外观正常黏膜的增殖活性进行分析。在结肠黏膜中，未观察到³H-胸腺嘧啶核苷标记指数（TLI）与样本距肿瘤的距离有显著差异。S期细胞沿隐窝长度的分布在底部较低，在下部25%范围内迅速增加并达到最大值，在最高位置则减少。当增殖活性增加时，增殖区有可能向隐窝的管腔区域扩展。将隐窝分为5部分，可以识别出2种不同模式：第一种模式是在下部五分之一处TLI非常高，然后急剧下降，在最高部分没有标记细胞；第二种模式是在管腔五分之一处也有标记细胞。这两个方面分别是TLI值最低和最高的标本的特征。对结直肠癌中TLI的分析表明，细胞动力学参数与临床和组织病理学特征如性别、年龄、Dukes分期和TNM分期以及分化程度无关。
