Eleutherio E C, Araujo P S, Panek A D
Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Brazil.
Cryobiology. 1993 Dec;30(6):591-6. doi: 10.1006/cryo.1993.1061.
Yeast cells accumulate the disaccharide trehalose in response to certain stress conditions. In an attempt to verify the role that trehalose plays when yeast cells are faced with heat stress, yeast mutant strains with specific lesions in trehalose metabolism were used. Cultures growing exponentially on glucose were shifted from 28 to 40 degrees C for 1 h. Accumulation of trehalose was correlated with heat tolerance, measured as resistance to 50.5 degrees C. Additionally, it was observed that the trehalose carrier was not involved in the mechanism of thermotolerance acquisition. Mutants that lack the carrier were also able to acquire thermotolerance as long as synthesis of the disaccharide took place.
酵母细胞在应对某些应激条件时会积累二糖海藻糖。为了验证海藻糖在酵母细胞面临热应激时所起的作用，使用了在海藻糖代谢中具有特定损伤的酵母突变菌株。在葡萄糖上指数生长的培养物从28℃转移到40℃ 1小时。海藻糖的积累与耐热性相关，耐热性以对50.5℃的抗性来衡量。此外，观察到海藻糖载体不参与耐热性获得机制。只要二糖的合成发生，缺乏载体的突变体也能够获得耐热性。
