Cleeter M W, Cooper J M, Darley-Usmar V M, Moncada S, Schapira A H
Department of Neuroscience, Royal Free Hospital School of Medicine, Hampstead, London, UK.
FEBS Lett. 1994 May 23;345(1):50-4. doi: 10.1016/0014-5793(94)00424-2.
Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the distal end of the respiratory chain. Analysis of the respiratory chain enzyme activities in the presence of GSNO localised the site of inhibition of cytochrome c oxidase alone. These results indicate that nitric oxide is capable of rapidly and reversibly inhibiting the mitochondrial respiratory chain and may be implicated in the cytotoxic effects of nitric oxide in the CNS and other tissues.
用一氧化氮供体S-亚硝基谷胱甘肽（GSNO）孵育大鼠骨骼肌线粒体，可使所有测试底物的氧利用受到可逆性抑制。受抑制线粒体的可见吸收光谱表明，细胞色素c+c1、b和a+a3被还原，这表明呼吸链远端存在阻断。在GSNO存在的情况下对呼吸链酶活性进行分析，结果表明仅细胞色素c氧化酶是抑制位点。这些结果表明，一氧化氮能够快速且可逆地抑制线粒体呼吸链，并且可能与一氧化氮在中枢神经系统和其他组织中的细胞毒性作用有关。
