Sugiyama S
Department of Radiology, Gunma University School of Medicine.
Kaku Igaku. 1994 Apr;31(4):327-36.
Basic study was performed to evaluate the efficacy of immunoscintigraphy of ovarian cancer using 99mTc labeled F(ab')2 fragments of monoclonal antibody OC125 which was reactive with CA125 antigen. Labeling efficiency was 99.9% and colloid formation was not observed. Specific activity was 1.48 GBq (40 mCi)/mg antibody and 99mTc OC125 preparation was stable more than 3 hr after labeling. Percent binding to 10(7) cells of PC-9 cancer cells expressing CA125 antigen was 47.0% in 99mTc OC125 almost the same as that of 125I OC125, Tumor-to-blood ratio for 99mTc OC125 was 5.73 +/- 1.41 at 18 hr after intravenous injection, which was significantly higher than that for 125I OC125. The scintigram showed a good localization in nude mouse bearing PC-9 tumor cells, and the clinical use of 99mTc OC125 is promising for the imaging of ovarian cancer.
进行基础研究以评估使用与CA125抗原反应的单克隆抗体OC125的99mTc标记F(ab')2片段进行卵巢癌免疫闪烁显像的疗效。标记效率为99.9%，未观察到胶体形成。比活度为1.48 GBq(40 mCi)/mg抗体，99mTc OC125制剂在标记后3小时以上保持稳定。在表达CA125抗原的PC-9癌细胞的10(7)个细胞上，99mTc OC125的结合百分比为47.0%，几乎与125I OC125相同。静脉注射后18小时，99mTc OC125的肿瘤与血液比值为5.73±1.41，明显高于125I OC125。闪烁图显示在携带PC-9肿瘤细胞的裸鼠中有良好的定位，99mTc OC125在临床上用于卵巢癌成像很有前景。
