Staunton J E, Weaver D T
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts.
Mol Cell Biol. 1994 Jun;14(6):3876-83. doi: 10.1128/mcb.14.6.3876-3883.1994.
The scid mouse mutation affects V(D)J rearrangement and double-strand break repair. scid V(D)J rearrangement is characterized by defective coding joint formation which prevents the development of mature B and T cells. Hairpin DNA has been implicated in the formation of V(D)J coding joints. We found scid cells to be proficient in hairpin processing in the context of DNA integration. In addition, we found that the scid defect did not impair integration of linear DNA via nonhomologous recombination. Therefore, hairpin processing and integration of DNA into the genome are distinct from hypersensitivity to ionizing radiation and the defect in V(D)J recombination.
严重联合免疫缺陷（scid）小鼠突变影响V（D）J重排和双链断裂修复。scid V（D）J重排的特征是编码接头形成缺陷，这阻止了成熟B细胞和T细胞的发育。发夹DNA与V（D）J编码接头的形成有关。我们发现scid细胞在DNA整合的情况下能够熟练地处理发夹。此外，我们发现scid缺陷并不损害线性DNA通过非同源重组的整合。因此，发夹处理和DNA整合到基因组中与对电离辐射的超敏反应和V（D）J重组缺陷不同。
