不依赖DNA同源性的V(D)J重组编码连接形成：编码末端的加工
V(D)J recombination coding junction formation without DNA homology: processing of coding termini.
作者信息
Boubnov N V, Wills Z P, Weaver D T
机构信息
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
出版信息
Mol Cell Biol. 1993 Nov;13(11):6957-68. doi: 10.1128/mcb.13.11.6957-6968.1993.
Abstract
Coding junction formation in V(D)J recombination generates diversity in the antigen recognition structures of immunoglobulin and T-cell receptor molecules by combining processes of deletion of terminal coding sequences and addition of nucleotides prior to joining. We have examined the role of coding end DNA composition in junction formation with plasmid substrates containing defined homopolymers flanking the recombination signal sequence elements. We found that coding junctions formed efficiently with or without terminal DNA homology. The extent of junctional deletion was conserved independent of coding ends with increased, partial, or no DNA homology. Interestingly, G/C homopolymer coding ends showed reduced deletion regardless of DNA homology. Therefore, DNA homology cannot be the primary determinant that stabilizes coding end structures for processing and joining.
摘要
V(D)J重组过程中编码连接的形成通过在连接前删除末端编码序列和添加核苷酸的过程，在免疫球蛋白和T细胞受体分子的抗原识别结构中产生多样性。我们利用含有位于重组信号序列元件侧翼的特定同聚物的质粒底物，研究了编码末端DNA组成在连接形成中的作用。我们发现，无论有无末端DNA同源性，编码连接都能高效形成。连接缺失的程度是保守的，与编码末端无关，DNA同源性增加、部分同源或无同源性时均如此。有趣的是，无论DNA同源性如何，G/C同聚物编码末端的缺失都减少。因此，DNA同源性不是稳定编码末端结构以进行加工和连接的主要决定因素。
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本文引用的文献
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