Tensen C P, Van Kesteren E R, Planta R J, Cox K J, Burke J F, van Heerikhuizen H, Vreugdenhil E
Department of Zoology, Graduate School of Neurosciences Amsterdam, Vrije Universiteit, Faculty of Biology, The Netherlands.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4816-20. doi: 10.1073/pnas.91.11.4816.
We have isolated and analyzed a cDNA from the central nervous system of the mollusc Lymnaea stagnalis encoding a putative receptor, which might be a natural hybrid between two different classes of receptor proteins. Preceded by a signal peptide, two types of repeated sequences are present in the N-terminal part of the protein. The first repeat displays a high sequence similarity to the extracellular binding domains of the low density lipoprotein receptor, which binds and internalizes cholesterol-containing apolipoproteins. The second repeat and the C-terminal part of the Lymnaea receptor are very similar to regions of a specific class of guanine nucleotide-binding protein-coupled receptors, the mammalian glycoprotein hormone receptors. The mRNA encoding the receptor is predominantly expressed in a small number of neurons within the central nervous system and to a lesser extent in the heart.
我们从椎实螺（Lymnaea stagnalis）的中枢神经系统中分离并分析了一种编码假定受体的cDNA，该受体可能是两类不同受体蛋白之间的天然杂交体。该蛋白的N端部分在信号肽之后存在两种重复序列。第一种重复序列与低密度脂蛋白受体的细胞外结合域具有高度序列相似性，低密度脂蛋白受体可结合并内化含胆固醇的载脂蛋白。椎实螺受体的第二种重复序列和C端部分与一类特定的鸟嘌呤核苷酸结合蛋白偶联受体（哺乳动物糖蛋白激素受体）的区域非常相似。编码该受体的mRNA主要在中枢神经系统内的少数神经元中表达，在心脏中的表达程度较低。
