Bauer G, O'Connell S, Devereux D, Martin A
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson University Hospital, New Brunswick.
Am Surg. 1994 Jun;60(6):455-9.
Triad is composed of Na pyruvate, vitamin E, and unsaturated fatty acids. We found that Triad, administered orally or topically, reverses wound healing that is impaired by doxorubicin (Doxo) in rats. Rats given Doxo (6 mg/kg i.v.) were wounded with linear dermal incisions, and the wound-breaking strength (WBS) was compared among groups of rats differently treated with Triad. Five groups of five rats each were studied. Triad was given orally using a 20 per cent Triad/rat chow mixture and topically as a 50 per cent Triad/petroleum base salve administered daily. All groups were wounded at postoperative day 0, at which time Doxo was given to groups II-IV. Group IV was fed oral Triad 7 days prior to wounding and until POD 21. All wounds were harvested at POD 21, and the mean WBS of each group was obtained using an Instron Tensiometer. Doxorubicin impaired normal wound healing by 40 per cent. Oral Triad restored WBS in Doxo-treated rats to 88 per cent of control values; topical Triad restored WBS to 80 per cent of control values. Moreover, treatment with topical Triad and oral Triad increased the WBS by 30 per cent and 50 per cent when compared with Doxo-only-treated animals. In conclusion, Triad has been shown to restore wound healing to nearly normal levels in doxorubicin-impaired wounds.
三联疗法由丙酮酸钠、维生素E和不饱和脂肪酸组成。我们发现，口服或局部使用三联疗法可逆转阿霉素（Doxo）所致大鼠伤口愈合受损的情况。给大鼠静脉注射阿霉素（6mg/kg）后，通过线性皮肤切口造成伤口，并比较不同三联疗法治疗组大鼠的伤口抗张强度（WBS）。每组5只大鼠，共研究5组。三联疗法口服采用20%三联疗法/大鼠饲料混合物，局部使用则为每日涂抹50%三联疗法/凡士林基质药膏。所有组均在术后第0天造成伤口，此时给II-IV组大鼠注射阿霉素。IV组在伤口形成前7天开始口服三联疗法，持续至术后第21天。所有伤口均在术后第21天采集，使用英斯特朗拉力试验机获得每组的平均WBS。阿霉素使正常伤口愈合受损40%。口服三联疗法可使阿霉素治疗的大鼠的WBS恢复至对照值的88%；局部使用三联疗法可使WBS恢复至对照值的80%。此外，与仅用阿霉素治疗的动物相比，局部和口服三联疗法分别使WBS提高了30%和50%。总之，三联疗法已被证明可使阿霉素所致受损伤口的愈合恢复至接近正常水平。
