茎菠萝蛋白酶的圆二色性：半胱氨酸蛋白酶家族中的第三种光谱类型。
Circular dichroism of stem bromelain: a third spectral class within the family of cysteine proteinases.
作者信息
Arroyo-Reyna A, Hernandez-Arana A, Arreguin-Espinosa R
机构信息
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, México.
出版信息
Biochem J. 1994 May 15;300 ( Pt 1)(Pt 1):107-10. doi: 10.1042/bj3000107.
Two forms of stem bromelain (EC 3.4.22.4) were isolated from commercial, crude and chromatographically purified preparations of the enzyme by means of gel-filtration and cation-exchange liquid chromatography. These forms possess nearly identical secondary and tertiary structures, as judged from their circular dichroism (c.d.) spectra. The spectral characteristics of stem bromelain suggest that this enzyme belongs to the alpha + beta protein class, as other cysteine proteinases do. In agreement with these results, quantitative estimation of secondary structures yielded amounts similar to those for papain and proteinase omega. However, the bromelain c.d. curve is clearly distinguishable from those reported for papain and proteinase omega, on one hand, and that of chymopapain, on the other. Thus, it is apparent that there are at least three types of c.d. spectra associated with the family of cysteine proteinases.
通过凝胶过滤和阳离子交换液相色谱法，从市售的粗制和色谱纯化的菠萝蛋白酶制剂（EC 3.4.22.4）中分离出两种形式的茎菠萝蛋白酶。从它们的圆二色性（c.d.）光谱判断，这些形式具有几乎相同的二级和三级结构。茎菠萝蛋白酶的光谱特征表明，这种酶与其他半胱氨酸蛋白酶一样，属于α+β蛋白类。与这些结果一致，二级结构的定量估计产生的量与木瓜蛋白酶和蛋白酶ω的量相似。然而，一方面，菠萝蛋白酶的c.d.曲线与报道的木瓜蛋白酶和蛋白酶ω的曲线明显不同，另一方面，与凝乳酶木瓜蛋白酶的曲线也明显不同。因此，很明显，至少有三种类型的c.d.光谱与半胱氨酸蛋白酶家族相关。
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