Sugaya R, Ishimaru S, Hosoya T, Saigo K, Emori Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Mech Dev. 1994 Feb;45(2):139-45. doi: 10.1016/0925-4773(94)90027-2.
We have identified a Drosophila gene encoding a putative receptor tyrosine kinase by screening a genomic DNA library with a DNA probe for a Drosophila homolog of fibroblast growth factor receptors. The newly isolated gene codes for a transmembrane protein most similar in sequence to a mammalian proto-oncogene ret; thus, the gene was termed Dret. Dret mRNA is transcribed in very small amounts in the embryonic, larval, and pupal stages. Whole mount in situ hybridization experiments revealed that the mRNA is transiently expressed in neuroblasts in early embryos. In late embryos, Dret mRNA was detected in subpopulations of differentiating CNS and PNS cells. In addition, Dret expression was affected in neurogenic mutants. These results suggest that Dret can be considered as a functional homolog of mammalian ret and should play important roles in neurogenesis.
我们通过用针对成纤维细胞生长因子受体的果蝇同源物的DNA探针筛选基因组DNA文库，鉴定出一个编码假定受体酪氨酸激酶的果蝇基因。新分离的基因编码一种跨膜蛋白，其序列与哺乳动物原癌基因ret最为相似；因此，该基因被命名为Dret。Dret mRNA在胚胎、幼虫和蛹阶段的转录量非常少。整体原位杂交实验表明，该mRNA在早期胚胎的神经母细胞中短暂表达。在晚期胚胎中，在分化的中枢神经系统和外周神经系统细胞亚群中检测到Dret mRNA。此外，Dret的表达在神经发生突变体中受到影响。这些结果表明，Dret可被视为哺乳动物ret的功能同源物，并且在神经发生中应发挥重要作用。
