Eastwood S L, Burnet P W, Harrison P J
University Department of Psychiatry, Warneford Hospital, Oxford, UK.
Neuroreport. 1994 Feb 24;5(6):677-80. doi: 10.1097/00001756-199402000-00004.
Synaptophysin is a presynaptic vesicle protein and a marker of synaptic density. We have studied the expression of its encoding mRNA in the brains of rats treated with haloperidol (2 mg kg-1 d-1) for two weeks. A significant increase in synaptophysin mRNA content was observed in the dorsolateral striatum but not in other brain areas compared with control animals. A similar trend was observed for synaptophysin immunoreactivity. Quantification of synaptophysin mRNA per cell showed that the increase was pronounced in large putatively cholinergic, striatal neurones. These data provide further evidence that localized synaptic plasticity occurs after neuroleptic treatment and indicate that such alterations are manifested in terms of expression of a synaptic protein gene by striatal neurones.
突触素是一种突触前囊泡蛋白，也是突触密度的标志物。我们研究了用氟哌啶醇（2毫克/千克/天）处理两周的大鼠大脑中其编码mRNA的表达情况。与对照动物相比，在背外侧纹状体中观察到突触素mRNA含量显著增加，但在其他脑区未观察到。突触素免疫反应性也观察到类似趋势。对每个细胞的突触素mRNA进行定量分析表明，在假定的大型胆碱能纹状体神经元中增加明显。这些数据进一步证明了抗精神病药物治疗后会发生局部突触可塑性，并表明这种改变表现为纹状体神经元中突触蛋白基因的表达。
