Becchi M, Aguilera R, Farizon Y, Flament M M, Casabianca H, James P
Service Central d'Analyse, CNRS, Vernaison, France.
Rapid Commun Mass Spectrom. 1994 Apr;8(4):304-8. doi: 10.1002/rcm.1290080404.
We propose a new confirmatory method for testosterone doping in sport. The present method in use, based on measuring the testosterone/epitestosterone (T/E) ratio in urine, may miss suspicious cases, or lead to reporting cases in which the high ratio is natural. Synthetic testosterone has a 13C abundance different from that of endogenous human testosterone. The connection of a gas chromatograph to an isotope-ratio mass spectrometer via a combustion interface allows the measurement of the corresponding characteristic value (delta /1000) for testosterone, its precursors, and its metabolites. To detect exogenous administration of testosterone, 30-40 mL of urine is sufficient.
我们提出了一种用于体育赛事中睾酮兴奋剂检测的新的确证方法。目前使用的方法是基于测量尿液中的睾酮/表睾酮（T/E）比值，该方法可能会遗漏可疑病例，或者导致报告一些高比值是自然情况的病例。合成睾酮的13C丰度与内源性人体睾酮不同。通过燃烧接口将气相色谱仪与同位素比值质谱仪相连，可测量睾酮及其前体和代谢物的相应特征值（δ/1000）。要检测外源性睾酮的使用，30 - 40毫升尿液就足够了。
