Vet Med (Praha). 1976 Mar;21(3):127-35.
The possibilities of utilizing the optimum conditions for revealing growth inhibiting substances in the carcasses of slaughtered animals were studied. A "direct" method (Kucera, 1969, 1970; Forschner, 1962) was used and operationally simple and usable methods of its application were searched for. The strains of Bacilus subtilis 9/59, Staphylococcus pyogenes aureus ATCC 6538, Bacilus cereus 4/58 were used for that diffusion method; the effect of sample size, duration of pre- diffusion, thickness and quality of agar, density and types of strains on the quality of zone were studied in renal and muscular tissue. The sample size did not affect, in a decisive manner, the quality of zone. On the other hand, the use of 18 hours old bouillon culture of the tester strain applied on agar with a swab was more advantageous than a swab smear from the plate culture. The duration of pre- diffusion and the application of ca. 1.4 mm thick agar had a significantly positive influence. Out of the three strains, Bacilus cereus proved the best catcher, Bacilus subtilis and Staphylococcus pyogenes aureus were less effective.
研究了利用最佳条件来揭示屠宰动物胴体中生长抑制物质的可能性。采用了一种“直接”方法（库采拉，1969年、1970年；福施纳，1962年），并寻找操作简单且实用的应用方法。将枯草芽孢杆菌9/59、金黄色化脓性葡萄球菌ATCC 6538、蜡样芽孢杆菌4/58菌株用于该扩散法；在肾脏和肌肉组织中研究了样品大小、预扩散持续时间、琼脂厚度和质量、菌株密度和类型对抑菌圈质量的影响。样品大小对抑菌圈质量没有决定性影响。另一方面，用拭子将18小时龄的测试菌株肉汤培养物接种到琼脂上比用平板培养物的拭子涂片更具优势。预扩散持续时间和使用约1.4毫米厚的琼脂有显著的积极影响。在这三种菌株中，蜡样芽孢杆菌被证明是最佳的捕捉菌，枯草芽孢杆菌和金黄色化脓性葡萄球菌的效果较差。
