Habrda J, Malíková M
Vet Med (Praha). 1976 Mar;21(3):167-74.
Streptomycin concentrations were determined in various calf tissues after i. m. administration of Streptomycin sulphate preparation of Rumanian provenience in the dose of l g pro toto using the biological diffusion method according to Gartside (1960). Spore suspensions of B. subtilis (ATCC 6633) and B. cereus var. mycoides (ATCC 11778) strains were employed as test organisms. B. subtilis was able to reveal considerably more residues. Another method using vegetative forms of the above microorganisms proved unsuitable for the tests. Two, three, five and ten days after the injection both slaughtered calves were positive, at 15 days only one of the two calves examined was positive, whereas at 20 days both calves were negative. Of the individual samples predominant majority of the findings come from the liver and from the spot of injection; residue findings from other tissues (bile, peritoneal muscles) are only sporadic. Skeletal musculature was negative in all cases except for the site of injection. In the samples from the injection site antimicrobial resudue activity was demonstrated in two cases following 1 month of freezing at -15 to -20 degrees C. For the tested preparation, a preslaughter withdrawal time of 20 days is recommended.
采用Gartside（1960年）的生物扩散法，以每头1克的剂量给小牛肌肉注射罗马尼亚产硫酸链霉素制剂后，测定了小牛各组织中的链霉素浓度。使用枯草芽孢杆菌（ATCC 6633）和蜡样芽孢杆菌蕈状变种（ATCC 11778）菌株的孢子悬浮液作为测试微生物。枯草芽孢杆菌能够检测到更多的残留。事实证明，使用上述微生物的营养体形式的另一种方法不适用于这些测试。注射后两天、三天、五天和十天，两只被宰杀的小牛检测结果均为阳性；在第15天，所检测的两只小牛中只有一只呈阳性，而在第20天，两只小牛均为阴性。在各个样本中，大多数检测结果来自肝脏和注射部位；其他组织（胆汁、腹膜肌肉）的残留检测结果只是零星出现。除注射部位外，所有情况下骨骼肌均为阴性。在注射部位的样本中，有两例在-15至-20摄氏度下冷冻1个月后仍显示出抗菌残留活性。对于所测试的制剂，建议屠宰前停药时间为20天。
