Hueck C, Kraus A, Hillen W
Institut für Mikrobiologie und Biochemie und Genetik, Universität Erlangen-Nürnberg, Germany.
Gene. 1994 May 27;143(1):147-8. doi: 10.1016/0378-1119(94)90621-1.
A regulatory gene with 69% nucleotide sequence identity to the Bacillus subtilis ccpA was cloned from Bacillus megaterium by complementation of a mutant relieved of catabolite repression. Sequencing of the gene and its adjacent regions revealed two additional open reading frames (ORFs) downstream from ccpA. These three genes are presumably in one operon. ORF1 and ORF2 show homology to two genes downstream from ccpA in B. subtilis, as well as to the B. subtilis motA and motB genes, respectively.
通过对一株解除分解代谢物阻遏的突变体进行互补，从巨大芽孢杆菌中克隆出了一个与枯草芽孢杆菌ccpA具有69%核苷酸序列同一性的调控基因。对该基因及其相邻区域进行测序后发现，ccpA下游还有另外两个开放阅读框（ORF）。这三个基因可能位于同一个操纵子中。ORF1和ORF2分别与枯草芽孢杆菌中ccpA下游的两个基因以及枯草芽孢杆菌的motA和motB基因具有同源性。
