干酪乳杆菌ATCC 393中的分解代谢物阻遏由CcpA介导。
Catabolite repression in Lactobacillus casei ATCC 393 is mediated by CcpA.
作者信息
Monedero V, Gosalbes M J, Pérez-Martínez G
机构信息
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Burjassot, Valencia, Spain.
出版信息
J Bacteriol. 1997 Nov;179(21):6657-64. doi: 10.1128/jb.179.21.6657-6664.1997.
Abstract
The chromosomal ccpA gene from Lactobacillus casei ATCC 393 has been cloned and sequenced. It encodes the CcpA protein, a central catabolite regulator belonging to the LacI-GalR family of bacterial repressors, and shows 54% identity with CcpA proteins from Bacillus subtilis and Bacillus megaterium. The L. casei ccpA gene was able to complement a B. subtilis ccpA mutant. An L. casei ccpA mutant showed increased doubling times and a relief of the catabolite repression of some enzymatic activities, such as N-acetylglucosaminidase and phospho-beta-galactosidase. Detailed analysis of CcpA activity was performed by using the promoter region of the L. casei chromosomal lacTEGF operon which is subject to catabolite repression and contains a catabolite responsive element (cre) consensus sequence. Deletion of this cre site or the presence of the ccpA mutation abolished the catabolite repression of a lacp::gusA fusion. These data support the role of CcpA as a common regulatory element mediating catabolite repression in low-GC-content gram-positive bacteria.
摘要
来自干酪乳杆菌ATCC 393的染色体ccpA基因已被克隆并测序。它编码CcpA蛋白，这是一种属于细菌阻遏物LacI-GalR家族的中心分解代谢调节因子，与枯草芽孢杆菌和巨大芽孢杆菌的CcpA蛋白具有54%的同一性。干酪乳杆菌ccpA基因能够互补枯草芽孢杆菌的ccpA突变体。干酪乳杆菌ccpA突变体显示出倍增时间增加，并且一些酶活性（如N-乙酰葡糖胺酶和磷酸-β-半乳糖苷酶）的分解代谢阻遏得到缓解。通过使用干酪乳杆菌染色体lacTEGF操纵子的启动子区域对CcpA活性进行了详细分析，该操纵子受到分解代谢阻遏，并且包含一个分解代谢物反应元件（cre）共有序列。该cre位点的缺失或ccpA突变的存在消除了lacp::gusA融合体的分解代谢阻遏。这些数据支持CcpA作为介导低GC含量革兰氏阳性细菌中分解代谢阻遏的常见调节元件的作用。
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Appl Environ Microbiol. 1991 Jun;57(6):1822-1828. doi: 10.1128/aem.57.6.1822-1828.1991.
2
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3
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J Mol Biol. 1997 Mar 7;266(4):665-76. doi: 10.1006/jmbi.1996.0820.
4
Establishing a model to study the regulation of the lactose operon in Lactobacillus casei.建立一个模型来研究干酪乳杆菌中乳糖操纵子的调控。
FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x.
5
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J Bacteriol. 1997 Mar;179(5):1555-62. doi: 10.1128/jb.179.5.1555-1562.1997.
6
Cyclic AMP-independent catabolite repression in bacteria.细菌中不依赖环磷酸腺苷的分解代谢物阻遏作用
FEMS Microbiol Lett. 1996 May 1;138(2-3):97-103. doi: 10.1111/j.1574-6968.1996.tb08141.x.
7
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J Bacteriol. 1996 Dec;178(23):7014-5. doi: 10.1128/jb.178.23.7014-7015.1996.
8
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Microbiology (Reading). 1996 Feb;142 ( Pt 2):217-230. doi: 10.1099/13500872-142-2-217.
9
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10
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