Hara S, Adachi Y, Kaneko Y, Fujimoto J, Hata J
Department of Pathology, National Children's Medical Research Center, Tokyo, Japan.
Br J Cancer. 1991 Dec;64(6):1025-30. doi: 10.1038/bjc.1991.458.
We have investigated the capability of differentiation of Ewing's sarcoma (ES) towards a neuronal direction through the establishment of four extraosseous ES cell lines and by in vitro stimulation with dibutyryladenosine cyclic monophosphate (db-cAMP) of eight ES lines. All except one of the lines expressed the molecule defined by 5C11, the antibody specifically reactive with ES. Two ES lines expressed a 200 kilodalton (kD) neurofilament protein (NFP) although their original tumours were negative for NFP. Elongation of cytoplasmic processes and increased NFP expression were observed after db-cAMP treatment of these lines and microtubules in the cytoplasmic processes were ultrastructurally demonstrated. Six lines were NFP negative, but three lines changed their morphology after induction of 200 kD NFP expression by db-cAMP treatment. The other three showed no definitive differentiation after db-cAMP treatment. Chromosomal analysis of the new ES lines showed the typical t(11;22) in one line and a +der(22) in two lines. No correlation was observed between the chromosomal abnormality and the differentiation capability. We conclude that ES is a heterogeneous group of tumours with respect to capability of differentiation into the neuronal lineage, but it is clearly distinguished from peripheral primitive neuroectodermal tumours by its 5C11 reactivity.
我们通过建立4个骨外尤文肉瘤（ES）细胞系，并对8个ES细胞系进行二丁酰环磷腺苷（db - cAMP）体外刺激，研究了尤文肉瘤向神经元方向分化的能力。除一个细胞系外，所有细胞系均表达由5C11所定义的分子，5C11是与ES特异性反应的抗体。两个ES细胞系表达一种200千道尔顿（kD）的神经丝蛋白（NFP），尽管其原发肿瘤NFP呈阴性。对这些细胞系进行db - cAMP处理后，观察到细胞质突起延长且NFP表达增加，并且在细胞质突起中通过超微结构证实了微管的存在。6个细胞系NFP呈阴性，但3个细胞系在经db - cAMP处理诱导200 kD NFP表达后形态发生了改变。另外3个细胞系在db - cAMP处理后未显示出明确的分化。对新的ES细胞系进行染色体分析，其中1个细胞系显示典型的t(11;22)，2个细胞系显示+der(22)。未观察到染色体异常与分化能力之间存在相关性。我们得出结论：就向神经元谱系分化的能力而言，ES是一组异质性肿瘤，但通过其5C11反应性可明显与外周原始神经外胚层肿瘤区分开来。
