大鼠肝癌发生过程中7H6紧密连接相关蛋白所揭示的紧密连接的顺序性降低。
Sequential decrease in tight junctions as revealed by 7H6 tight junction-associated protein during rat hepatocarcinogenesis.
作者信息
Zhong Y, Enomoto K, Tobioka H, Konishi Y, Satoh M, Mori M
机构信息
Department of Pathology, Sapporo Medical University School of Medicine.
出版信息
Jpn J Cancer Res. 1994 Apr;85(4):351-6. doi: 10.1111/j.1349-7006.1994.tb02366.x.
Abstract
A sequential decrease in the number of hepatocyte tight junctions during the course of rat hepatocarcinogenesis was demonstrated by immunohistochemistry with a new 7H6 monoclonal antibody generated in our laboratory. Semiquantitative analysis by confocal laser scanning microscopy revealed that the expression of 7H6 antigen was reduced in hyperplastic foci, hyperplastic nodules and hepatocellular carcinomas (HCC) to 43%, 28% and 25%, respectively, compared to corresponding normal liver tissues. 7H6 antigen was scarce in HCC with a trabecular pattern, whereas it was expressed intensely at the apical and basolateral membrane of HCC with a glandular pattern. Immunoblot analysis of 7H6 expression in hepatocellular carcinomas showed a decrease roughly coincident with that shown by immunohistochemistry. These results indicated, for the first time, that tight junctions decrease progressively during carcinogenesis, leading to disruption of cellular polarity and cellular adhesiveness.
摘要
利用我们实验室新制备的7H6单克隆抗体进行免疫组织化学检测，结果显示在大鼠肝癌发生过程中，肝细胞紧密连接的数量呈序贯性减少。通过共聚焦激光扫描显微镜进行的半定量分析表明，与相应的正常肝组织相比，7H6抗原在增生灶、增生结节和肝细胞癌（HCC）中的表达分别降至43%、28%和25%。7H6抗原在小梁状模式的HCC中稀少，而在腺样模式的HCC的顶端和基底外侧膜强烈表达。对肝细胞癌中7H6表达的免疫印迹分析显示其下降情况与免疫组织化学结果大致相符。这些结果首次表明，紧密连接在致癌过程中逐渐减少，导致细胞极性和细胞黏附性的破坏。
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