Blethen S L, Albertsson-Wikland K, Faklis E J, Chasalow F I
Department of Pediatrics, State University of New York, Stony Brook 11794-8111.
J Clin Endocrinol Metab. 1994 Jun;78(6):1439-43. doi: 10.1210/jcem.78.6.8200948.
To evaluate the presence of different GH isoforms in serum of girls with Turner's syndrome, we measured the serum GH content using RIAs with three different site-specific monoclonal antibodies (MAbs). We compared the results to those obtained with authentic GH and GH isoforms. Compared to pituitary GH (mol wt, 22K daltons) as the standard for all three MAbs, serum from girls with Turner's syndrome did not displace tracer [125I]GH equally with all three MAbs. The relative amounts of GH-immunoreactive material found in Turner's syndrome were different from the amounts observed in normal adults and most children with idiopathic short stature. The presence of GH, other than 22K GH, in serum from girls with Turner's syndrome was confirmed by affinity chromatography. The existence of different isoforms of GH, as shown by different cross-reactivity patterns with different MAbs to GH, may explain the conflicting results reported for GH secretion in girls with Turner's syndrome.
为评估特纳综合征女孩血清中不同生长激素（GH）异构体的存在情况，我们使用针对三种不同位点特异性单克隆抗体（MAb）的放射免疫分析法（RIA）测定了血清GH含量。我们将结果与使用 authentic GH和GH异构体获得的结果进行了比较。与垂体GH（分子量22千道尔顿）作为所有三种MAb的标准相比，特纳综合征女孩的血清并非与所有三种MAb同等程度地置换示踪剂[125I]GH。在特纳综合征中发现的GH免疫反应性物质的相对量与正常成年人以及大多数特发性身材矮小儿童中观察到的量不同。通过亲和层析证实了特纳综合征女孩血清中除22K GH之外还存在其他GH。不同的GH异构体与不同的GH MAb具有不同的交叉反应模式，这可能解释了关于特纳综合征女孩GH分泌的报道结果相互矛盾的原因。
