Henderson J T, Seniuk N A, Richardson P M, Gauldie J, Roder J C
Samuel Lunenfeld Research Institute, Division of Neurobiology and Molecular Immunology, Mount Sinai Hospital, Toronto, Ontario, Canada.
J Clin Invest. 1994 Jun;93(6):2632-8. doi: 10.1172/JCI117276.
Ciliary neurotrophic factor (CNTF) has previously been shown to promote the survival of several classes of neurons and glial. We report here that in addition to its effects on the nervous system, CNTF can induce potent effects in extra-neural tissues. Implantation of C6 glioma cells engineered to secrete CNTF either subcutaneously or into the peritoneal cavity of adult mice, or systemic injections of purified rat or human recombinant CNTF, resulted in a rapid syndrome of weight loss resulting in death over a period of 7-10 d. This weight loss could not be explained by a reduction in food intake and involved losses of both fat and skeletal muscle. CNTF also induced the synthesis of acute phase proteins such as haptoglobin. Implantation of C6 lines expressing a nonsecreted form of CNTF, or the parental C6 line itself, did not result in wasting effects. Analysis of this CNTF-induced wasting indicates similarities with the previously described cachectins, tumor necrosis factor, interleukin 6, and leukemia inhibitory factor, but does not involve the induction of these cytokines.
睫状神经营养因子（CNTF）此前已被证明可促进几类神经元和神经胶质细胞的存活。我们在此报告，除了对神经系统有作用外，CNTF还能在神经外组织中诱导显著效应。将经基因工程改造可分泌CNTF的C6胶质瘤细胞皮下植入成年小鼠或注入成年小鼠腹腔，或全身注射纯化的大鼠或人重组CNTF，会导致迅速的体重减轻综合征，在7至10天内导致死亡。这种体重减轻不能用食物摄入量减少来解释，涉及脂肪和骨骼肌的损失。CNTF还诱导了急性期蛋白如触珠蛋白的合成。植入表达非分泌形式CNTF的C6细胞系或亲本C6细胞系本身，不会导致消瘦效应。对这种由CNTF诱导的消瘦的分析表明，它与先前描述的恶病质素、肿瘤坏死因子、白细胞介素6和白血病抑制因子有相似之处，但不涉及这些细胞因子的诱导。
