Laboratory of Histology Embryology and Cytogenetic (UR 12 ES 10), Faculty of Medicine, University of Monastir, Street Avicenne, 5019, Monastir, Tunisia.
Laboratory of Cytogenetics and Reproductive Biology, Center of Maternity and Neonatology, Monastir, Fattouma Bourguiba University Teaching Hospital, Monastir, Tunisia.
Environ Sci Pollut Res Int. 2019 May;26(14):14097-14105. doi: 10.1007/s11356-019-04575-7. Epub 2019 Mar 9.
The present study aimed to determine the level of iron and calcium in the seminal plasma of men with different fertility potentials and to examine its relationship with oxidative stress. Seventy-nine sub-fertile patients with asthenoteratozoospermia (AT), n 27; teratoleucozoospermia (TL), n 20; teratozoospermia (Terato), n 32; and 29 healthy donors were included. The ability of spermatozoa to produce reactive oxygen species (ROS) was evaluated by using nitroblue tetrazolium (NBT) staining. The lipid peroxidation end product, malondialdehyde (MDA), and the trace element levels (iron and calcium) were measured spectrophotometrically. Iron and calcium concentrations in seminal plasma of the patient groups were significantly more elevated than the normal group. Nevertheless, both calcium and iron showed strong negative correlations with the total sperm motility and normal sperm morphology, but only iron was positively and significantly associated with multiple anomalies index and seminal leucocyte concentration. On the other hand, the rates of MDA and ROS production in semen were significantly higher in the three abnormal groups than in controls. These two oxidative stress biomarkers were significantly associated with the percentage of atypical forms in semen. However, only semen ROS level was significantly associated with the decreased sperm motility and the sperm leucocytes concentration. Meanwhile, there are positive correlations between seminal iron and calcium content and the studied oxidative stress biomarkers. Oxidative stress and trace element excess are implicated in low sperm quality. Iron and calcium might be the mediators of the effects of oxidative damage and induces lipid peroxidation.
本研究旨在确定不同生育能力男性精液中铁和钙的水平，并研究其与氧化应激的关系。纳入 79 例弱精症患者，其中包括少精弱精症（AT）27 例、畸形精子症（TL）20 例、畸形精子症（Terato）32 例，以及 29 名健康供体。通过硝基四氮唑蓝（NBT）染色评估精子产生活性氧（ROS）的能力。采用分光光度法测定脂质过氧化终产物丙二醛（MDA）和微量元素（铁和钙）水平。患者组精液中铁和钙浓度明显高于正常组。然而，钙和铁均与总精子活力和正常精子形态呈强负相关，但只有铁与多形性指数和精液白细胞浓度呈正显著相关。另一方面，三组异常组精液中 MDA 和 ROS 的产生率明显高于对照组。这两个氧化应激生物标志物与精液中非典型形态的百分比显著相关。然而，只有精液 ROS 水平与精子活力降低和精子白细胞浓度显著相关。同时，精液中铁和钙含量与研究中的氧化应激生物标志物呈正相关。氧化应激和微量元素过多与精子质量低有关。铁和钙可能是氧化损伤和诱导脂质过氧化作用的介质。
