Sato N, Deckelbaum R J, Neeser G, Carpentier Y A, Kinney J M
Department of Pediatrics, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
JPEN J Parenter Enteral Nutr. 1994 Mar-Apr;18(2):112-8. doi: 10.1177/0148607194018002112.
We explored the effects of plasma-like conditions on hydrolysis of medium-chain triglyceride (MCT) and long-chain triglyceride (LCT) emulsions at different mixing ratios and the effect of the physical method of mixing on lipoprotein lipase hydrolysis of mixed emulsions in vitro. Mixed emulsions with two different mixing ratios, 50% MCTs with 50% LCTs and 70% MCTs with 30% LCTs by weight, were studied. Emulsions containing both MCT and LCT oils blended in the same emulsion particle were compared with mixtures of separate pure MCT emulsion particles and pure LCT particles. MCT hydrolysis was always greater than LCT hydrolysis. In a plasma-free tris(hydroxymethyl)aminomethane-albumin buffer at pH 8.5, the physical method of mixing had substantial effects on hydrolysis; blended emulsions of MCTs and LCTs were hydrolyzed better than separate mixes of pure MCT and pure LCT particles, ie, more total free fatty acids were released. In plasma-free systems, there were no differences in rates of hydrolysis of LCTs or MCTs (as individual triglycerides) between the two different mixing ratios of 50:50 and 70:30. However, the presence of plasma markedly diminished the differences in hydrolysis between blended vs separately mixed emulsions at pH 7.4. Also, in plasma-like incubation buffer, the rates of hydrolysis of MCTs and LCTs in emulsions with 50:50 or 70:30 MCT to LCT ratios reflected the respective amounts of MCT and LCTs in the emulsions.(ABSTRACT TRUNCATED AT 250 WORDS)
我们探讨了类似血浆的条件对不同混合比例的中链甘油三酯（MCT）和长链甘油三酯（LCT）乳剂水解的影响，以及体外混合物理方法对混合乳剂脂蛋白脂肪酶水解的影响。研究了两种不同混合比例的混合乳剂，按重量计分别为50% MCT与50% LCT以及70% MCT与30% LCT。将在同一乳剂颗粒中混合了MCT和LCT油的乳剂与单独的纯MCT乳剂颗粒和纯LCT颗粒的混合物进行比较。MCT的水解总是大于LCT的水解。在pH 8.5的无血浆三（羟甲基）氨基甲烷 - 白蛋白缓冲液中，混合的物理方法对水解有显著影响；MCT和LCT的混合乳剂比纯MCT和纯LCT颗粒的单独混合物水解得更好，即释放出更多的总游离脂肪酸。在无血浆系统中，50:50和70:30这两种不同混合比例的LCT或MCT（作为单个甘油三酯）的水解速率没有差异。然而，血浆的存在显著减小了pH 7.4时混合乳剂与单独混合乳剂之间水解的差异。此外，在类似血浆的孵育缓冲液中，MCT与LCT比例为50:50或70:30的乳剂中MCT和LCT的水解速率反映了乳剂中MCT和LCT的各自含量。（摘要截选至250字）
