Serizawa H, Miura S, Tashiro H, Imaeda H, Shiozaki H, Ohkubo N, Kimura H, Tanaka S, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
JPEN J Parenter Enteral Nutr. 1994 Mar-Apr;18(2):141-7. doi: 10.1177/0148607194018002141.
The effects of an elemental diet on lymphocyte transport in intestinal lymph and immune responses of gut-associated lymphoid tissue were investigated in rats. The control animals were fed a conventional diet. After 4 week of feeding, the total calorie intake and body weight gain showed no differences between the two groups. The number and total area of Peyer's patches and the ratio of height of villi to height of crypt showed no significant differences between the two groups. The rate of lymph flow in intestinal lymphatics showed no significant change in treated animals compared with the control rats. However, an elemental diet induced a significant decrease in lymphocyte flux in intestinal lymphatics compared with that in control rats. Lymphocyte subsets in intestinal lymph revealed a significant decrease in CD3-positive cells, especially CD4-positive cells in the elemental diet-treated group. A significant decrease in the number of immunoglobulin A-containing cells and a decreased CD4/CD8 ratio in T-cell subsets were observed in the lamina propria of ileal mucosa in the elemental diet-treated group by morphometric analysis in the immunohistochemical study. Specific antibody-secreting cells in intestinal lymph were also investigated after rats were intraduodenally primed with cholera toxin and challenged with the same toxin after an interval of 2 weeks. No significant difference was seen between the two groups in any of the numbers of anti-cholera toxin immunoglobulin-secreting cells in any immunoglobulin A, immunoglobulin G, or immunoglobulin M class as determined by the enzyme-linked immunospot assay.(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠中研究了要素饮食对肠淋巴中淋巴细胞转运及肠道相关淋巴组织免疫反应的影响。对照动物喂食常规饮食。喂食4周后，两组的总卡路里摄入量和体重增加无差异。两组间派尔集合淋巴结的数量和总面积以及绒毛高度与隐窝高度之比均无显著差异。与对照大鼠相比，处理组动物肠淋巴管中的淋巴流速无显著变化。然而，与对照大鼠相比，要素饮食导致肠淋巴管中淋巴细胞通量显著降低。肠淋巴中的淋巴细胞亚群显示，要素饮食处理组中CD3阳性细胞显著减少，尤其是CD4阳性细胞。在免疫组织化学研究中，通过形态计量分析观察到，要素饮食处理组回肠黏膜固有层中含免疫球蛋白A的细胞数量显著减少，T细胞亚群中CD4/CD8比值降低。在大鼠经十二指肠用霍乱毒素致敏并在2周间隔后用相同毒素攻击后，还研究了肠淋巴中特异性抗体分泌细胞。通过酶联免疫斑点测定法测定，两组间任何免疫球蛋白A、免疫球蛋白G或免疫球蛋白M类中抗霍乱毒素免疫球蛋白分泌细胞的数量均无显著差异。（摘要截短于250字）
