Ohkubo N, Miura S, Serizawa H, Yan H J, Kimura H, Imaeda H, Tashiro H, Tsuchiya M
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan.
Regul Pept. 1994 Feb 24;50(2):127-35. doi: 10.1016/0167-0115(94)90028-0.
The in vivo effects of chronic administration of vasoactive intestinal peptides (VIP) on the lymphoid cell traffic and the population and function of cells in intestinal lymph and gut-associated lymphoid tissues were examined in rats. VIP was continuously infused from the superior mesenteric artery in rats at a dose of 10 ng/min/kg body weight for 96 h. Lymphocyte transport through intestinal lymph was significantly reduced by VIP without any changes in lymph flow. When lymphocyte subpopulation was examined in intestinal lymph, T cell subsets were decreased with a dominant reduction in the population of helper T cells. T cell subsets were also decreased in mesenteric lymph nodes, but in this case suppressor/cytotoxic T cell subsets were mainly reduced. Despite of the decrease in lymphocyte transport through intestinal lymph and changes of lymphocyte subpopulation, proliferative response of lymphocytes from intestinal lymph and mesenteric lymph nodes to phytohemagglutinin did not show any significant alteration after administration of VIP. By histochemical study on the lamina propria of the small intestine, the population of pan T cells, especially helper T cells, was demonstrated to be significantly decreased after VIP treatment. There was also a marked decrease in the number of immunoglobulin (Ig) A-containing cells in the lamina propria of the small intestine in VIP-treated rats, while no significant changes were seen in the number of IgG and IgM-containing cells. Our present results showed the possibility that a long-term alteration of serum VIP levels could affect the dynamics of immune effector cells and IgA production in gut-associated lymphoid tissues.
研究了慢性给予血管活性肠肽（VIP）对大鼠淋巴样细胞运输以及肠淋巴液和肠道相关淋巴组织中细胞群体及功能的体内效应。以10 ng/分钟/千克体重的剂量从大鼠肠系膜上动脉持续输注VIP 96小时。VIP显著降低了淋巴细胞通过肠淋巴液的运输，而淋巴液流量无任何变化。在检测肠淋巴液中的淋巴细胞亚群时，T细胞亚群减少，其中辅助性T细胞群体减少占主导。肠系膜淋巴结中的T细胞亚群也减少，但在这种情况下，主要是抑制性/细胞毒性T细胞亚群减少。尽管通过肠淋巴液的淋巴细胞运输减少以及淋巴细胞亚群发生变化，但给予VIP后，来自肠淋巴液和肠系膜淋巴结的淋巴细胞对植物血凝素的增殖反应未显示任何显著改变。通过对小肠固有层的组织化学研究表明，VIP处理后泛T细胞群体，尤其是辅助性T细胞，显著减少。VIP处理的大鼠小肠固有层中含免疫球蛋白（Ig）A的细胞数量也显著减少，而含IgG和IgM的细胞数量未见明显变化。我们目前的结果表明，血清VIP水平的长期改变可能影响肠道相关淋巴组织中免疫效应细胞的动态变化和IgA的产生。
