Okamoto K, Sekito T, Yoshida K
Department of Biological Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Mol Gen Genet. 1994 May 25;243(4):473-6. doi: 10.1007/BF00280478.
Genes homologous to those encoding mitochondrial NADH dehydrogenase subunits ND4L and ND5 in filamentous fungi were identified in the mitochondrial genome of a budding yeast, Hansenula wingei. The structure and expression of these genes were investigated. The H. wingei ND4L gene is 282 bp long, and potentially codes for a polypeptide of 94 amino acids. The putative ND4L protein sequence shares about 46% homology with the analogous mitochondrial proteins of filamentous fungi. The H. wingei ND5 gene is 1935 bp long, and encodes a 645-residue polypeptide. The derived ND5 protein shares about 38% sequence homology with the analogue in filamentous fungi. The ND4L and ND5 genes have no intervening sequence, and form a gene cluster in the order of 5'-ND4L-ND5-3'. A presumptive mature dicistronic or polycistronic transcript of these genes was detected by Northern blot hybridization. These results strongly indicate that these ND4L and ND5 genes are active. As far as we are aware, this is the first report on the identification of mitochondrially encoded ND genes in yeast.
在一种芽殖酵母——汉逊酵母（Hansenula wingei）的线粒体基因组中，鉴定出了与丝状真菌中编码线粒体NADH脱氢酶亚基ND4L和ND5的基因同源的基因。对这些基因的结构和表达进行了研究。汉逊酵母的ND4L基因长度为282 bp，可能编码一个由94个氨基酸组成的多肽。推测的ND4L蛋白序列与丝状真菌的类似线粒体蛋白具有约46%的同源性。汉逊酵母的ND5基因长度为1935 bp，编码一个由645个残基组成的多肽。推导的ND5蛋白与丝状真菌中的类似物具有约38%的序列同源性。ND4L和ND5基因没有间隔序列，按5'-ND4L-ND5-3'的顺序形成一个基因簇。通过Northern印迹杂交检测到了这些基因推测的成熟双顺反子或多顺反子转录本。这些结果有力地表明这些ND4L和ND5基因是有活性的。据我们所知，这是关于酵母中线粒体编码的ND基因鉴定的首次报道。
