Okamoto K, Suzuki K, Yoshida K
Botanical Institute, Faculty of Science, Hiroshima University, Higashi, Japan.
Jpn J Genet. 1991 Dec;66(6):709-18. doi: 10.1266/jjg.66.709.
Mitochondrial DNAs from the ascosporogenous yeasts S. exiguus, S. kluyveri and H. wingei were prepared by a new rapid method without CsCl isopycnic centrifugation. The mtDNA RFLPs were identified and clearly distinguished each species from the other. The physical maps were constructed by single and double digestion with nine restriction endonucleases. The location of rRNA genes was assigned to the maps by Southern hybridization with synthetic consensus probes. The genome sizes of these mtDNAs were estimated to be 45 kb for S. exiguus, 54 kb for S. kluyveri and 27 kb for H. wingei. The mtDNA RFLP analysis indicates a phylogenetic relationship among these yeasts. This indicates that S. cerevisiae is closer to H. wingei than S. kluyveri. However, the derived phylogenetic tree is completely consistent with that which was previously constructed on amino acid replacement in mating pheromones and electrophoretic karyotypes (Yoshida et al., 1989).
通过一种无需氯化铯等密度离心的新型快速方法，制备了来自产囊酵母矮小毕赤酵母、克鲁维毕赤酵母和温氏汉逊酵母的线粒体DNA。鉴定了线粒体DNA的限制性片段长度多态性（RFLPs），并能清晰地区分每个物种。通过用9种限制性内切酶进行单酶切和双酶切构建了物理图谱。通过与合成共有探针的Southern杂交，将核糖体RNA（rRNA）基因的位置标注到图谱上。这些线粒体DNA的基因组大小估计分别为：矮小毕赤酵母45 kb，克鲁维毕赤酵母54 kb，温氏汉逊酵母27 kb。线粒体DNA的RFLP分析表明了这些酵母之间的系统发育关系。这表明酿酒酵母与温氏汉逊酵母的亲缘关系比与克鲁维毕赤酵母更近。然而，由此得出的系统发育树与先前基于交配信息素中的氨基酸替换和电泳核型构建的系统发育树完全一致（吉田等人，1989年）。
