Moore M S, Kuenzel W J, Mench J A
Department of Poultry Science, University of Maryland, College Park 20742.
Poult Sci. 1994 Apr;73(4):556-63. doi: 10.3382/ps.0730556.
The measurement of central levels of neurochemicals is an important approach to the understanding of the neurophysiological basis of behavior patterns in animals. Previous studies have utilized central sampling techniques developed for individually housed animals. The purpose of this study was to develop a cannulation technique and a method for sampling cerebrospinal fluid (CSF) from socially housed birds to facilitate the study of the neurophysiological basis of social behaviors. The cannulation technique involved the surgical implantation of a 22-gauge concentric guide cannula into the cisterna magna of 16-wk-old, feed-restricted male broiler breeders (n = 6). Individual-specific coordinates and optimum angle and depth of implantation of the cannula were determined in order to place the cannula correctly in the designated site. Once implanted, the guide cannula proved to be unobtrusive and secure and did not attract aggressive pecking from other birds in the pen. Two methods of CSF sampling were then examined. The first method required the use of a push-pull perfusion pump to withdraw CSF at a rate of 1 to 2 microL/min. The second method (passive), which did not use a pump, involved simply removing a "dummy" cannula from the guide cannula to release the CSF, which was then collected with a glass Hamilton syringe. Samples ranging from 100 to 500 microL were collected using the passive method. The combination of the cannulation technique described and the passive sampling method proved to be the most simple, efficient, and reliable method for measuring central levels of neurochemicals in socially housed broiler breeder males.
测量神经化学物质的中枢水平是理解动物行为模式神经生理基础的重要方法。先前的研究采用了为单独饲养的动物开发的中枢采样技术。本研究的目的是开发一种插管技术和从群居鸟类中采集脑脊液（CSF）的方法，以促进对社会行为神经生理基础的研究。插管技术包括将一根22号同心导向插管手术植入16周龄、限饲的雄性肉种鸡（n = 6）的枕大池。确定了个体特异性坐标以及插管植入的最佳角度和深度，以便将插管正确放置在指定位置。一旦植入，导向插管被证明不显眼且牢固，不会引起围栏中其他鸟类的攻击性啄击。然后研究了两种脑脊液采样方法。第一种方法需要使用推挽式灌注泵以1至2微升/分钟的速度抽取脑脊液。第二种方法（被动法）不使用泵，只需从导向插管中取出一个“假”插管以释放脑脊液，然后用玻璃汉密尔顿注射器收集。使用被动法收集了100至500微升的样本。所描述的插管技术与被动采样方法相结合，被证明是测量群居雄性肉种鸡神经化学物质中枢水平最简单、最有效和最可靠的方法。
