Bouman H J, Van Wimersma Greidanus T B
Brain Res Bull. 1979 Jul-Aug;4(4):575-7. doi: 10.1016/0361-9230(79)90045-5.
A cannulation technique for frequent sampling of cerebrospinal fluid (CSF) in unanaesthetized freely moving rats is described. A permanent stainless steel cannula, constructed in such a way that no loss of CSF occurs, is placed into the rat's cisterna magna and fixed to the skull by anchoring screws and dental cement. A special CSF outflow opening of the cannula is connected to polyethylene tubing for CSF sampling. Amounts of 50-150 microliters CSF can be collected repeatedly without any sign of disturbing the animal. The technique lends itself not only to pilot studies in which within a short period of time a large amount of CSF is wanted, but also to experiments in which physiological conditions are required.
本文描述了一种用于在未麻醉的自由活动大鼠中频繁采集脑脊液（CSF）的插管技术。制作了一种永久性不锈钢插管，其构造可防止脑脊液流失，将其置于大鼠的枕大池，并通过固定螺钉和牙科用水泥固定在颅骨上。插管上有一个特殊的脑脊液流出开口，连接到用于采集脑脊液的聚乙烯管上。可以反复采集50 - 150微升的脑脊液，而不会出现任何干扰动物的迹象。该技术不仅适用于短期内需要大量脑脊液的初步研究，也适用于需要生理条件的实验。
