Costa C, Rinaldi A M, Romancino D P, Cavalcante C, Vizzini A, Di Carlo M
Istituto di Biologia dello Sviluppo C.N.R., Palermo, Italy.
FEBS Lett. 1997 Jun 30;410(2-3):499-501. doi: 10.1016/s0014-5793(97)00641-8.
Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.
