Di Carlo M, Romancino D P, Ortolani G, Montana G, Giudice G
Istituto di Biologia dello Sviluppo C.N.R. Palermo, Italy.
Biochem Biophys Res Commun. 1996 Dec 13;229(2):511-7. doi: 10.1006/bbrc.1996.1835.
Microsurgery experiments demonstrate that the animal side of the unfertilized sea urchin Paracentrotus lividus egg coincides with the side of the egg pronucleus location. It is demonstrated by means of in situ hybridization and immunostaining of whole mounts of animal or vegetal halves that the previously identified bep 1 and bep4 RNAs and their proteins are located in the animal part of the unfertilized egg and much less in the vegetal part. The addition of Fabs against BEP1 and BEP4 causes exogastrulation.
