Tzipori S
Aust J Exp Biol Med Sci. 1975 Aug;53(4):273-9. doi: 10.1038/icb.1975.30.
Bovine ephemeral fever (BEF) virus was isolated from infected fresh cattle blood directly into Vero cell cultures. One cycle of rapid freeze-thaw destroyed the infectivity of the virus to Vero cells. The infectivity to baby mice by intracerebral inoculation, on the other hand, was only reduced. The occurrence of autointerference due to the presence of defective interfering particles in cell culture was also noted. BEF virus strain 919 was propagated to some extent in bovine kidney, testis and synovial cell monolayers, producing cytopathic effect (CPE) in 24 h. However, the CPE was nonprogressive and the addition of antinomycin D to the medium improved the virus titre only slightly.
牛暂时热（BEF）病毒从感染的新鲜牛血液中直接分离出来接种到Vero细胞培养物中。一轮快速冻融破坏了病毒对Vero细胞的感染性。另一方面，通过脑内接种对乳鼠的感染性仅有所降低。还注意到细胞培养物中由于缺陷干扰颗粒的存在而发生自身干扰。BEF病毒株919在牛肾、睾丸和滑膜细胞单层中能一定程度地增殖，24小时内产生细胞病变效应（CPE）。然而，CPE不进展，向培养基中添加放线菌素D仅使病毒滴度略有提高。
