Elamin M A, Spradbrow P B
J Hyg (Lond). 1978 Aug;81(1):1-7. doi: 10.1017/s0022172400053705.
Unadapted bovine ephemeral fever (BEF) virus was isolated from cattle blood after intravenous inoculation into chicken embryos. Infected embryos died or hatched as abnormal chickens. The chick embryo was slightly less sensitive to unadapted BEF virus than were Vero cell cultures, but the use of embryos avoids the several blind passages that are required to isolate BEF virus in unweaned mice. Chick embryos were considerably less efficient than Vero cell culture or unweaned mice in detecting Vero cell-adapted and mouse-adapted BEF virus respectively. Viraemia was demonstrated in chicken embryos at 1-4 days and in one-day-old chickens at 1-3 days after intravenous inoculation of BEF virus. BEF virus was demonstrated by isolation and by immunofluoresence in heart, brain, lung and liver of chicken embryos at 1-5 days and in lung and liver of one-day-old chickens at 1-2 days, after intravenous inoculation. The isolated viruses were confirmed as BEF virus by neutralization with immune mouse ascitic fluid. BEF neutralizing antibodies were produced in 4-week-old and adult chickens after intravenous inoculation with BEF virus.
将未适应的牛流行热（BEF）病毒静脉接种到鸡胚后，从牛血液中分离出该病毒。受感染的胚胎死亡或孵出异常鸡。鸡胚对未适应的BEF病毒的敏感性略低于Vero细胞培养物，但使用鸡胚可避免在未断奶小鼠中分离BEF病毒所需的多次盲传。在分别检测Vero细胞适应型和小鼠适应型BEF病毒时，鸡胚的效率远低于Vero细胞培养或未断奶小鼠。静脉接种BEF病毒后，在1至4天的鸡胚以及1至3天的一日龄鸡中均检测到病毒血症。静脉接种后，在1至5天的鸡胚以及1至2天的一日龄鸡的心脏、脑、肺和肝脏中，通过病毒分离和免疫荧光法检测到BEF病毒。用免疫小鼠腹水进行中和试验，证实分离出的病毒为BEF病毒。静脉接种BEF病毒后，4周龄和成年鸡产生了BEF中和抗体。
